Role of enterocyte stearoyl-Co-A desaturase-1 in LDLR-null mice.

After crossing floxed stearoyl-CoA desaturase-1 () mice with LDL receptor-null () mice, and then Villin Cre () mice, enterocyte expression in // mice was reduced 70%. On Western diet (WD), / mice gained more weight than // mice ( < 0.0023). On WD, jejunum levels of lysophosphatidylcholine (LysoPC) 18:1 and lysophosphatidic acid (LPA) 18:1 were significantly less in // compared with / mice ( < 0.0004 and < 0.026, respectively). On WD, // mice compared with / mice had lower protein levels of lipopolysaccharide-binding protein (LBP), cluster of differentiation 14 (CD14), toll-like receptor 4 (TLR4), and myeloid differentiation factor-88 (MyD88) in enterocytes and plasma, and less dyslipidemia and systemic inflammation. Adding a concentrate of tomatoes transgenic for the apoA-I mimetic peptide 6F (Tg6F) to WD resulted in reduced enterocyte protein levels of LBP, CD14, TLR4, and MyD88 in / mice similar to that seen in // mice. Adding LysoPC 18:1 to WD did not reverse the effects of enterocyte knockdown. Adding LysoPC 18:1 (but not LysoPC 18:0) to chow induced jejunum expression and increased dyslipidemia and plasma serum amyloid A and interleukin 6 levels in / mice, but not in // mice. We conclude that enterocyte is partially responsible for LysoPC 18:1- and WD-induced dyslipidemia and inflammation in mice.