Guan Wei-Jie, Yuan Jing-Jing, Li Hui-Min, Gao Yong-Hua, Chen Chun-Lan, Huang Yan, Chen Rong-Chang, Zhong Nan-Shan
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China,
Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou, People's Republic of China,
Int J Chron Obstruct Pulmon Dis. 2018 Jul 17;13:2173-2182. doi: 10.2147/COPD.S159335. eCollection 2018.
Bronchiectasis is a debilitating disease with chronic airway infection. , the dominant phylum, can be detected with high-throughput sequencing.
To stratify compositions according to culture findings in bronchiectasis.
We sampled sputum, split for culture and 16srRNA sequencing, from 106 patients with stable bronchiectasis and 17 healthy subjects. Paired sputa from 22 bronchiectasis patients were collected during exacerbations and convalescence.
Forty-five, 41, and 20 patients with clinically stable bronchiectasis had isolated (PA), other potentially pathogenic microorganisms, and commensals at the initial visit, respectively. The PA group (but not other groups) demonstrated significantly greater relative abundance of , and lower Shannon-Wiener Diversity Index, Simpson Diversity Index, and richness compared with healthy subjects. was the dominant genus that discriminated bronchiectasis patients (particularly in the PA group) from healthy subjects. Compared with baseline levels, community compositions in the PA group, but not in other groups, were more resilient during exacerbations and convalescence.
community compositions could be partially reflected by conventional sputum bacterial culture. Significantly altered community compositions - particularly, the increased relative abundance of and diminished community diversity - represent critical targets for novel interventions to restore normal airway microen-vironment in patients with bronchiectasis.
支气管扩张症是一种伴有慢性气道感染的使人衰弱的疾病。通过高通量测序可以检测到作为优势菌门的[具体菌门名称未给出]。
根据支气管扩张症的培养结果对[具体微生物名称未给出]菌群组成进行分层。
我们从106例稳定期支气管扩张症患者和17名健康受试者中采集痰液，将其分为用于培养和16srRNA测序的样本。在病情加重期和恢复期收集了22例支气管扩张症患者的配对痰液。
在初次就诊时，45例、41例和20例临床稳定的支气管扩张症患者分别分离出[具体细菌名称未给出]（PA）、其他潜在致病微生物和共生菌。与健康受试者相比，PA组（而非其他组）显示出[具体细菌名称未给出]的相对丰度显著更高，而香农 - 维纳多样性指数、辛普森多样性指数和丰富度更低。[具体细菌名称未给出]是区分支气管扩张症患者（特别是PA组）与健康受试者的优势菌属。与基线水平相比，PA组而非其他组的[具体微生物名称未给出]菌群组成在病情加重期和恢复期更具弹性。
传统痰液细菌培养可部分反映[具体微生物名称未给出]菌群组成。显著改变的[具体微生物名称未给出]菌群组成——特别是[具体细菌名称未给出]相对丰度增加和菌群多样性降低——是恢复支气管扩张症患者气道微环境正常的新型干预措施的关键靶点。
