Rae B P, Elliott R M
J Gen Virol. 1986 Jul;67 ( Pt 7):1351-60. doi: 10.1099/0022-1317-67-7-1351.
A full length cDNA copy of the NS mRNA of the Missouri strain (Hazelhurst subtype, New Jersey serotype) of vesicular stomatitis virus (VSV) has been cloned and sequenced. The mRNA is 856 nucleotides long (excluding polyadenylic acid) and encodes a protein of 274 amino acids (mol. wt. 31 000). Comparison with the NS gene of the Ogden strain (Concan subtype, New Jersey serotype) showed 15% difference at the nucleotide level and 10% difference at the amino acid level; the majority of the changes were located in the 3' half of the mRNA. Comparison with the NS genes of two strains representing the Indiana serotype showed about 50% nucleotide and 33% amino acid sequence homology between the serotypes. In a four-way comparison of the proteins, two regions of higher homology were noted which may be of functional importance. Eighteen potential phosphorylation sites (Ser or Thr) were conserved between the four proteins; five of these sites correspond to the residues which have been suggested to be constitutively phosphorylated and may be essential for NS activity.
水疱性口炎病毒（VSV）密苏里株（黑兹尔赫斯特亚型，新泽西血清型）的NS mRNA全长cDNA拷贝已被克隆并测序。该mRNA长856个核苷酸（不包括聚腺苷酸），编码一个含274个氨基酸的蛋白质（分子量31000）。与奥格登株（康坎亚型，新泽西血清型）的NS基因比较显示，核苷酸水平上有15%的差异，氨基酸水平上有10%的差异；大多数变化位于mRNA的3'端一半。与代表印第安纳血清型的两个毒株的NS基因比较显示，血清型之间约有50%的核苷酸和33%的氨基酸序列同源性。在对这四种蛋白质的四向比较中，发现了两个同源性较高的区域，可能具有功能重要性。这四种蛋白质之间有18个潜在的磷酸化位点（丝氨酸或苏氨酸）是保守的；其中五个位点对应于已被认为是组成型磷酸化的残基，可能对NS活性至关重要。
