Eiden M, Trainor C D, Reitz M S
J Gen Virol. 1986 Jul;67 ( Pt 7):1455-60. doi: 10.1099/0022-1317-67-7-1455.
Fibroblast cell lines infected in vitro with different strains of gibbon ape leukaemia virus or the related woolly monkey virus (SSAV) synthesized two RNA species of approximately 8.4 kb and 2.9 kb. The former, a complete RNA, represents the gag-pol mRNA, while the latter is a spliced transcript lacking gag and pol, and represents the env mRNA. In contrast, RNA from one T-lymphoid cell line derived from a gibbon ape T-lymphocytic leukaemia (UCD-144) expressed a viral mRNA in addition to gag-pol and env mRNA. This RNA is 6.4 kb and lacks at least 3.0 kb of sequences derived from the internal region of the viral genome, including most or all of the pol gene. These data, as well as data from Southern blots of UCD-144 DNA, suggest that the 6.4 kb mRNA could represent a transcript from a defective recombinant provirus and may contain cell-derived sequences.
在体外被不同株长臂猿白血病病毒或相关绒毛猴病毒（SSAV）感染的成纤维细胞系合成了两种大小约为8.4 kb和2.9 kb的RNA。前者是完整RNA，代表gag-pol mRNA，而后者是缺少gag和pol的剪接转录本，代表env mRNA。相比之下，来自一只长臂猿T淋巴细胞白血病（UCD-144）的一个T淋巴细胞系的RNA除了表达gag-pol和env mRNA外，还表达一种病毒mRNA。这种RNA为6.4 kb，缺少至少3.0 kb源自病毒基因组内部区域的序列，包括大部分或全部pol基因。这些数据以及UCD-144 DNA的Southern印迹数据表明，6.4 kb mRNA可能代表来自缺陷重组原病毒的转录本，并且可能包含细胞来源的序列。
