Satter S, Mahbub H, Shamsuzzaman S M
Dr Sarmin Satter, Assistant Professor, Department of Microbiology, CARe Medical College, Dhaka, Bangladesh; E-mail:
Mymensingh Med J. 2018 Jul;27(3):561-566.
This cross-sectional study was conducted in the Department of Microbiology of Dhaka Medical College, Dhaka, Bangladesh, from January 2015 to December 2015 to investigate the antibiotic resistance pattern and prevalence of genes encoding aminoglycoside-modifying enzymes in Escherichia coli and Klebsiella species isolated from a tertiary care hospital of Bangladesh. A total of 166 Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca were isolated from urine, wound swab, pus, sputum and blood samples of patients of Dhaka Medical College Hospital. Antibiotic susceptibility test was performed by disk-diffusion technique. Genes encoding aminoglycoside-modifying enzymes [aac(3)-IIa, aac(6')-Ib, ant(2'')-Ia, aac(3)-Ia] among the isolated Escherichia coli and Klebsiella species were detected by polymerase chain reaction. The most prevalent aminoglycoside-modifying enzyme gene found was aac (3)-IIa (60.24%), followed by aac(6')-Ib (57.23%), ant(2'')-Ia (12.05%) and aac(3)-Ia (10.84%). Colistin and tigecycline were the most effective antibacterial agents against aminoglycoside-modifying enzymes followed by imipenem. The results of this study showed high proportion of aminoglycoside-modifying enzymes producing Escherichia coli and Klebsiella species in Bangladesh.
本横断面研究于2015年1月至2015年12月在孟加拉国达卡达卡医学院微生物学系进行，旨在调查从孟加拉国一家三级护理医院分离出的大肠杆菌和克雷伯菌属中抗生素耐药模式以及编码氨基糖苷类修饰酶的基因的流行情况。从达卡医学院医院患者的尿液、伤口拭子、脓液、痰液和血液样本中总共分离出166株大肠杆菌、肺炎克雷伯菌和产酸克雷伯菌。采用纸片扩散法进行抗生素敏感性试验。通过聚合酶链反应检测分离出的大肠杆菌和克雷伯菌属中编码氨基糖苷类修饰酶的基因[aac(3)-IIa、aac(6')-Ib、ant(2'')-Ia、aac(3)-Ia]。发现最常见的氨基糖苷类修饰酶基因是aac(3)-IIa（60.24%），其次是aac(6')-Ib（57.23%）、ant(2'')-Ia（12.05%）和aac(3)-Ia（10.84%）。黏菌素和替加环素是对抗氨基糖苷类修饰酶最有效的抗菌剂，其次是亚胺培南。本研究结果表明，在孟加拉国，产生氨基糖苷类修饰酶的大肠杆菌和克雷伯菌属比例很高。
