Roi A A, Kisten A G, Tsarenko I Yu, Kurdish I K
Mikrobiol Z. 2016 May-Jun;78(3):78-87.
The features of the growth of Azotobacter vinelandii IMV V-7076 and Bacillus subtilis IMV V-7023 in optimized environments in single and mixed culture at deep cultivation to simulate the accumulation stage (capacity) biomass biotechnology creation of complex bacterial preparation for crop production. When growing Bacillus subtilis IMV B-7023 in monoculture specific growth rate of the bacteria was higher than that of Azotobacter vinelandii IMV B-7076. Therefore, by cultivating a mixed culture medium in the fermenter was inoculated with the bacterial suspension A. vinelandii + B. subtilis in a ratio of 10 : 1. The greatest biomass accumulation was observed after 24 hours of cultivation of mixed cultures. Spent submerged culture A. vinelandii IMV B-7076 and B. subtilis IMV B-7023 in the fermenter can be recommended for suspension of high-viable cells of both strains in biotechnology industrial manufacturing complex bacterial preparation for crop production.
在深层培养的优化环境中，对棕色固氮菌IMV V-7076和枯草芽孢杆菌IMV V-7023进行单培养和混合培养，以模拟生物量积累阶段（能力），为作物生产创建复合细菌制剂的生物技术。当枯草芽孢杆菌IMV B-7023进行单培养时，细菌的比生长速率高于棕色固氮菌IMV B-7076。因此，在发酵罐中以10:1的比例接种棕色固氮菌+枯草芽孢杆菌的细菌悬液来培养混合培养基。混合培养物培养24小时后观察到最大的生物量积累。发酵罐中用过的棕色固氮菌IMV B-7076和枯草芽孢杆菌IMV B-7023的深层培养物可推荐用于生物技术工业生产中这两种菌株高活力细胞的悬浮，以制备用于作物生产的复合细菌制剂。
