Dorneanu Olilvia Simona, Luncă Cătălina, Năstase E V, Tuchiluş Cristina Gabriela, Vremeră Teodora, Iancu Luminiţa Smaranda
Rev Med Chir Soc Med Nat Iasi. 2016 Oct-Dec;120(4):886-91.
Methicillin-resistant Staphylococcus aureus can become resistant to many different classes of antibiotics. Objective: To characterize aminoglycoside and macrolide resistance mechanisms in MRSA strains in relation to antibiotic susceptibility patterns. Materials and methods: We tested 86 MRSA strains using multiplex PCR for detection of genes mecA, aac(6′)-Ie/aph(2″), ant(4′)-Ia, aph(3′)-IIIa, ermA, ermC and msrA. Results: There was a prevalence of msrA (32.5%), ermC (30.2%) and aph(3')-IIIa (61.6%) genes, which are less frequently reported in MRSA. Most msrA genes was detected in PVL positive strains (92.8%) and was associated only with non-MDR strains, while ermC genes were associated with MDR strains. PVL producing strains were characterized by the presence of aph(3′)-IIIa (93.1%) and msrA genes (93.1%), being phenotypically susceptible to clindamycin. Conclusions: Detection of aminoglycoside and macrolide resistance genes allowed us to establish the concordance between genotypic and phenotypic methods and to correlate the presence of certain resistance genes with the type of circulating strain and the production of virulence factors.
耐甲氧西林金黄色葡萄球菌可对许多不同类别的抗生素产生耐药性。目的：根据抗生素敏感性模式，对耐甲氧西林金黄色葡萄球菌（MRSA）菌株中的氨基糖苷类和大环内酯类耐药机制进行表征。材料与方法：我们使用多重聚合酶链反应（PCR）检测了86株MRSA菌株，以检测mecA、aac(6′)-Ie/aph(2″)、ant(4′)-Ia、aph(3′)-IIIa、ermA、ermC和msrA基因。结果：msrA（32.5%）、ermC（30.2%）和aph(3')-IIIa（61.6%）基因普遍存在，这些基因在MRSA中报道较少。大多数msrA基因在Panton-Valentine杀白细胞素（PVL）阳性菌株中检测到（92.8%），且仅与非多重耐药（MDR）菌株相关，而ermC基因与MDR菌株相关。产PVL菌株的特征是存在aph(3′)-IIIa（93.1%）和msrA基因（93.1%），其表型对克林霉素敏感。结论：氨基糖苷类和大环内酯类耐药基因的检测使我们能够确定基因型和表型方法之间的一致性，并将某些耐药基因的存在与流行菌株类型和毒力因子的产生相关联。
