Faculty of Medicine, Department of Ophthalmology, Kırıkkale University , Kırıkkale , Turkey.
Faculty of Medicine, Department of Dermatovenereology, Kırıkkale University , Kırıkkale , Turkey.
Ocul Immunol Inflamm. 2019;27(7):1174-1183. doi: 10.1080/09273948.2018.1504971. Epub 2018 Aug 24.
: To investigate the role of innate immunity in ocular rosacea. : Thirty-two patients with ocular rosacea patients (group-1) and 28 healthy volunteers (group-2) who served as controls were enrolled in the study. Tear function parameters were assessed, conjunctival impression cytology was performed and tear samples were collected. Human-neutrophil-peptides (HNP) 1-3 and human-beta-defensin-2 (hBD-2) levels were measured in tears by using ELISA tests. Cathelicidin leucin-leucin-37 (LL-37), hBD-2, human-beta-defensin-9 (hBD-9) gene expression levels were measured in the conjunctival impression cytology samples using real-time polymerase chain reaction. : Tear HNP1-3 ( = 0.024), hBD-2 ( < 0.001), conjunctival LL-37 gene expression rate ( = 0.014) and ocular surface disease index scores ( = 0.001) were higher and the tear break-up time was lower ( = 0.003) in group-1. No other differences were found between the groups. : The results of this study suggest the role of abnormal innate immunity in the pathophysiology of ocular rosacea by revealing elevated antimicrobial peptide levels.
: 目的：探讨固有免疫在眼型酒渣鼻中的作用。: 本研究纳入 32 例眼型酒渣鼻患者（组 1）和 28 名健康志愿者（组 2）作为对照。评估泪液功能参数，进行结膜印迹细胞学检查并收集泪液标本。采用 ELISA 法检测泪液中人中性粒细胞肽（HNP）1-3 和人β防御素-2（hBD-2）水平。采用实时聚合酶链反应检测结膜印迹细胞学样本中cathelicidin leucin-leucin-37（LL-37）、hBD-2、人β防御素-9（hBD-9）基因表达水平。: 组 1 的泪液 HNP1-3（=0.024）、hBD-2（<0.001）、结膜 LL-37 基因表达率（=0.014）和眼表疾病指数评分（=0.001）较高，泪膜破裂时间较短（=0.003）。两组间无其他差异。: 本研究结果提示异常固有免疫在眼型酒渣鼻的病理生理学中发挥作用，其特征为抗菌肽水平升高。
