Inouye S, Hattori K, Yuki S, Saigo K
Nucleic Acids Res. 1986 Jun 25;14(12):4765-78. doi: 10.1093/nar/14.12.4765.
More than 21 members of 17.6, a Drosophila retrotransposon, were isolated and their possible structural changes were examined by restriction mapping, blot hybridization, heteroduplex analysis and nucleotide sequence determination of long terminal repeats (LTRs). At least 7 members were found to suffer with terminal or internal long deletions. No pair of LTRs having an identical nucleotide sequence was found either within an element or between elements. Although an initiation site for the presumable genome-sized transcript of 17.6, a potential substrate for reverse transcription on translocation, was identified within the left-hand LTR, our results as a whole support the notion that the majority of 17.6s have continued to reside for a long period of time at their present chromosomal loci and hence the rate of translocation of 17.6 is very low.
从果蝇反转录转座子17.6中分离出了超过21个成员，并通过限制性酶切图谱分析、印迹杂交、异源双链分析以及长末端重复序列（LTR）的核苷酸序列测定，研究了它们可能的结构变化。发现至少7个成员存在末端或内部的长片段缺失。在一个元件内部或元件之间，均未发现一对具有相同核苷酸序列的LTR。尽管在左侧LTR内确定了17.6推测的基因组大小转录本的起始位点，而该转录本是易位时反转录的潜在底物，但我们的整体结果支持这样一种观点，即大多数17.6长期以来一直位于其当前的染色体位点，因此17.6的易位率非常低。
