Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, 1955 East-West Road, Honolulu, HI 96822, USA.
Department of Molecular Biosciences and Bioengineering, University of Hawai'i at Mānoa, 1955 East-West Road, Honolulu, HI 96822, USA.
Biotechnol Adv. 2018 Nov 15;36(7):1971-1983. doi: 10.1016/j.biotechadv.2018.08.007. Epub 2018 Aug 23.
Exposure of a small amount of oxygen/air (microaeration) has been reported to benefit the anaerobic digestion (AD) process in enhancing hydrolysis, improving methane yield, stabilizing the process and scavenging hydrogen sulfide among others. The underlying mechanism of enhancing AD process via microaeration is the augmentation of activity and diversity of the microbial consortia that promotes syntrophic interactions among different microbial groups, thereby creating a more stable process. To design and implement a microaeration-based AD process, fundamental insights about the mechanism of the AD system at process, microbial and molecular levels must be fully explored. This review critically examines microaeration-based AD processes through our recent understandings of the effect of oxygen on microbial community structure, enzymatic, energetic, physiological, and biochemical aspects of the microbial-mediated process. Syntrophic interactions between hydrolytic, fermentative, sulfate reducing, syntrophic bacteria and methanogens under microaerobic conditions are examined to reveal putative mechanism and factors that need to be considered when implementing microaeration in AD process. Further studies are needed to better understand the microbial pathways and bioenergetics of the microaerobic AD process by adopting advanced molecular techniques such as metagenomics, transcriptomics, and proteomics.
据报道，少量氧气/空气（微曝气）的暴露有益于厌氧消化（AD）过程，通过提高水解作用、提高甲烷产量、稳定过程和清除硫化氢等方式来改善该过程。通过微曝气增强 AD 过程的潜在机制是增强微生物群落的活性和多样性，促进不同微生物群体之间的共生相互作用，从而创造更稳定的过程。为了设计和实施基于微曝气的 AD 工艺，必须充分探索 AD 系统在工艺、微生物和分子水平上的机制。本综述通过我们对氧气对微生物群落结构、酶、能量、生理和微生物介导过程的生化方面的影响的最新认识，批判性地检查了基于微曝气的 AD 工艺。研究了在微氧条件下水解、发酵、硫酸盐还原、产甲烷菌和产甲烷菌之间的共生相互作用，以揭示在 AD 过程中实施微曝气时需要考虑的潜在机制和因素。需要进一步的研究来更好地了解采用宏基因组学、转录组学和蛋白质组学等先进分子技术的微需氧 AD 过程的微生物途径和生物能量学。
