Nascimento Amanda Fiorentina, Kirychuk Shelley, Bernardi Maria Martha, Felício Luciano F
Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Department of Medicine/Canadian Centre for Health & Safety in Agriculture (CCHSA), University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Neuroimmunomodulation. 2018;25(2):89-95. doi: 10.1159/000490894. Epub 2018 Aug 24.
Previous studies from our group showed that lipopolysaccharide (LPS) exposure induces several signs of sickness behavior, including a decrease in food consumption, body weight gain, adipsia, and a biphasic effect in tympanic temperature with a first phase of hypothermia, followed by an increased tympanic temperature. LPS can activate a chain of nonspecific host responses, including the immune response, and decreased zinc levels. In addition, there are differences in the immune response between males and females, particularly fever, with sex hormones interfering with body temperature. This study aims to characterize the effects of zinc treatment on tympanic temperature, body weight gain, food and water consumption, and general activity in open field of virgin female rats exposed to a dose of LPS that was previously reported to induce sickness behavior.
Virgin female Wistar rats were treated with either saline (S) or LPS. One hour later, the S group received another injection of saline (S + S group), half of the LPS group received saline (LPS + S group) and the other half received zinc (LPS + Zn group). Tympanic temperature, body weight, and water and food consumption were measured for 96 h. Measurements and observations started 2 h after LPS administration.
Treatment with zinc attenuated LPS-increased temperature, decreased the body weight gain and food consumption, and water consumption was increased.
Zinc treatment is beneficial as it reduces the increased tympanic temperature induced by LPS, but it does not influence other sickness behavior caused by exposure to LPS.
我们团队之前的研究表明，暴露于脂多糖（LPS）会引发多种疾病行为迹象，包括食物摄入量减少、体重增加、烦渴，以及鼓膜温度呈现双相变化，第一阶段体温过低，随后鼓膜温度升高。LPS可激活一系列非特异性宿主反应，包括免疫反应，并降低锌水平。此外，雄性和雌性之间的免疫反应存在差异，尤其是发热，性激素会干扰体温。本研究旨在表征锌治疗对暴露于先前报道可诱发疾病行为剂量LPS的未孕雌性大鼠鼓膜温度、体重增加、食物和水消耗以及旷场中的一般活动的影响。
将未孕雌性Wistar大鼠分为生理盐水（S）组或LPS组进行处理。1小时后，S组再注射一次生理盐水（S + S组），LPS组一半大鼠接受生理盐水注射（LPS + S组），另一半接受锌注射（LPS + Zn组）。在96小时内测量鼓膜温度、体重以及水和食物的消耗量。测量和观察在给予LPS后2小时开始。
锌治疗减轻了LPS引起的体温升高，减少了体重增加和食物消耗，且水消耗量增加。
锌治疗有益，因为它降低了LPS诱导的鼓膜温度升高，但不影响暴露于LPS引起的其他疾病行为。
