Pref-1 及其相关趋化因子在大鼠肠系膜淋巴结发育过程中的表达。

Expression of Pref-1 and Related Chemokines during theDevelopment of Rat Mesenteric Lymph Nodes.

机构信息

Department of Anatomy, Basic Medical Science College, Harbin Medical University, Harbin 150081, Heilongjiang, China.

Department of Pharmacology, Pharmacy College, Harbin Medical University, Harbin 150081, Heilongjiang, China.

出版信息

Biomed Environ Sci. 2018 Jul;31(7):507-514. doi: 10.3967/bes2018.068.

DOI:10.3967/bes2018.068

PMID:30145985

Abstract

OBJECTIVE

The aim of this study was to investigate the ability of Pref-1+ adipocyte progenitor cells to mobilize into mesenteric lymph nodes (MLNs) and the dynamic expression of related chemokines during the development of rat MLNs.

METHODS

Immunohistochemical analyses were used to detect the expression of Pref-1 and related chemokines. Transmission electron microscopy (TEM) was used to observe the changes in ultrastructure of MLNs.

RESULTS

Cells containing lipid droplets were found in all rat MLNs at embryonic day (E) 18.5, 2 and 6 weeks (w) after birth, and they were similar to fibroblastic reticular cells (FRCs) or follicular dendritic cells (FDCs) under TEM. Pref-1+ adipocyte progenitor cells were found in all MLNs. The expression level of Pref-1 was significantly increased at 2 w after birth and decreased at 6 w after birth. The tendency of Cxcl12 expression was consistent with that of Pref-1 and was positively correlated with the expression of Pref-1 (P < 0.01; r = 0.897). At E18.5, Cxcl13, and Ccr7 were significantly expressed in the MLN anlage, but the expression level of Ccl21 was low. The expression level of Cxcl13, Ccr7, and Ccl21 in MLN were significantly increased at 2 w after birth (P < 0.05), while the expression of Ccr7 and Ccl21 were significantly decreased at 6 w after birth (P < 0.05).

CONCLUSION

Adipocyte progenitor cells are involved in the rat MLNs development through differentiation into FRC and FDC. The expression of the relevant chemokines during the development of MLNs is dynamic and may be related to the maintenance of lymph nodes self-balance state.

摘要

目的

本研究旨在探讨 Pref-1+脂肪前体细胞向肠系膜淋巴结（MLNs）动员的能力，以及在大鼠 MLNs 发育过程中相关趋化因子的动态表达。

方法

采用免疫组织化学分析检测 Pref-1 及相关趋化因子的表达。透射电镜（TEM）观察 MLNs 超微结构的变化。

结果

胚胎第 18.5 天（E）、出生后 2 周（w）和 6 周（w）的所有大鼠 MLNs 中均发现含有脂滴的细胞，其在 TEM 下类似于纤维母细胞网状细胞（FRC）或滤泡树突状细胞（FDC）。所有 MLNs 中均发现 Pref-1+脂肪前体细胞。出生后 2w 时 Pref-1 表达水平显著升高，6w 时降低。Cxcl12 表达趋势与 Pref-1 一致，与 Pref-1 呈正相关（P < 0.01；r = 0.897）。E18.5 时，MLN 原基中 Cxcl13 和 Ccr7 表达明显，而 Ccl21 表达水平较低。出生后 2w，MLN 中 Cxcl13、Ccr7 和 Ccl21 的表达水平显著升高（P < 0.05），而 Ccr7 和 Ccl21 的表达水平在出生后 6w 时显著降低（P < 0.05）。