Schlegel R, Wade-Glass M, Rabson M S, Yang Y C
Science. 1986 Jul 25;233(4762):464-7. doi: 10.1126/science.3014660.
Bovine papillomavirus (BPV-1) contains two independent transforming genes that have been mapped to the E5 and E6 open reading frames (ORF's). The E5 transforming protein was identified by means of an antiserum against a synthetic peptide corresponding to the 20 COOH-terminal amino acids of the E5 ORF. The E5 polypeptide is the smallest viral transforming protein yet characterized; it had an apparent size of 7 kilodaltons. The transforming polypeptide is encoded entirely within the second half of the E5 ORF and its predicted amino acid composition is very unusual; 68% of the amino acids are strongly hydrophobic and 34% are leucine. Cell fractionation studies localized this polypeptide predominantly to cellular membranes.
牛乳头瘤病毒（BPV - 1）含有两个独立的转化基因，已被定位到E5和E6开放阅读框（ORF）。E5转化蛋白是通过针对与E5 ORF的20个COOH末端氨基酸相对应的合成肽的抗血清鉴定出来的。E5多肽是迄今已鉴定的最小的病毒转化蛋白；其表观大小为7千道尔顿。转化多肽完全在E5 ORF的后半部分编码，其预测的氨基酸组成非常不寻常；68%的氨基酸是强疏水性的，34%是亮氨酸。细胞分级分离研究将该多肽主要定位在细胞膜上。
