Hou Yan-Long, You Chong-Ge
Clin Lab. 2018 Jul 1;64(7):1113-1119. doi: 10.7754/Clin.Lab.2018.180318.
High-resolution melting curve analysis has been widely applied as one of the mainstream technologies used for scanning and detecting genetic mutations. After having realized the integration of PCR amplification and HRM detection on the same instrument, researchers have begun to focus on the throughput and speed of PCR-HRM detection.
A systematic literature search was conducted with the use of PubMed and ScienceDirect for articles on high resolution melting curve and microfluidic technologies. The focus is on the manufacture and application of microfluidic devices in medical diagnostic. A further review of the references identified from these articles was also performed.
Based on the characteristics of microfluidic integration and rapid detection of microfluidic technology, a new high-throughput PCR-HRM genotyping and scanning detection mode combining microfluidic technology has not only achieved closed Tube high-throughput rapid detection, but also meets the needs of portability and lowcost.
The new PCR-HRM detection platform based on the microfluidic technology can achieve high throughput and high speed.
高分辨率熔解曲线分析已作为用于扫描和检测基因突变的主流技术之一被广泛应用。在实现了PCR扩增和HRM检测在同一仪器上的整合后，研究人员开始关注PCR-HRM检测的通量和速度。
利用PubMed和ScienceDirect对有关高分辨率熔解曲线和微流控技术的文章进行系统的文献检索。重点是微流控装置在医学诊断中的制造和应用。还对从这些文章中识别出的参考文献进行了进一步审查。
基于微流控集成的特点和微流控技术的快速检测，一种结合微流控技术的新型高通量PCR-HRM基因分型和扫描检测模式不仅实现了闭管高通量快速检测，而且满足了便携性和低成本的需求。
基于微流控技术的新型PCR-HRM检测平台能够实现高通量和高速度。
