Desselberger U, Hung T, Follett E A
Virus Res. 1986 Jun;4(4):357-68. doi: 10.1016/0168-1702(86)90082-1.
The genomes of adult diarrhoea rotaviruses isolated in different parts of China during winter outbreaks in 1983 and 1984 were compared by segmental oligonucleotide (ON) mapping. The RNA profiles of most of the isolates were indistinguishable but it was found that some corresponding RNA segments had identical or very closely related ON maps whereas others differed considerably. This finding can be taken to suggest that the strains compared may be genetically related by a natural reassortment event. The genomes of cocirculating group A rotaviruses isolated in Scotland during winter outbreaks in 1981/82 were also compared. The ON maps of corresponding RNA segment differed extensively irrespective of whether or not the segments comigrated on gels.
采用分段寡核苷酸(ON)图谱法,对1983年和1984年冬季中国不同地区爆发的成人腹泻轮状病毒的基因组进行了比较。大多数分离株的RNA图谱无法区分,但发现一些相应的RNA片段具有相同或非常相近的ON图谱,而其他片段则有很大差异。这一发现表明,所比较的毒株可能通过自然重配事件存在遗传关系。还对1981/82年冬季苏格兰爆发期间共循环的A组轮状病毒的基因组进行了比较。无论片段在凝胶上是否共迁移,相应RNA片段的ON图谱都有很大差异。