Tungjai Montree, Sukantamala Singhadeth, Malasaem Pimchanok, Dechsupa Nathupakorn, Kothan Suchart
The Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
Toxicol Rep. 2018 Aug 16;5:840-845. doi: 10.1016/j.toxrep.2018.08.012. eCollection 2018.
This study reveals the antioxidant properties of iodinated radiographic contrast media to be used in diagnostic radiology. Di(phenyl)-(2,4,6-trinitrophenyl) iminoazanium (DPPH), ferric reducing ability of plasma (FRAP), and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assays were used for determining the antioxidant properties of five iodinated radiographic contrast media such as iobitridol (xenetix), iodixanol (visipaque), iohexol (omnipaque), ioxaglate (hexabrix), and isovue (iopamiro). An ascorbic acid and Trolox solution served as a positive control. The absorbance intensity of the colored product was recorded using a spectrophotometer. For DPPH and ABTS assay, the absorbance intensity at 533 and 752 nm, respectively was decreased when compared to control; it indicated an increase in antioxidant activity. For FRAP assay, the absorbance intensity at 593 nm was increased when compared to control; it indicated an increase in antioxidant activity. The results showed that five iodinated radiographic contrast media did not differ in DPPH radical-scavenging activity when compared to a corresponding control. The ferric reducing ability of all of these iodinated radiographic contrast media also did not differ when compared to a corresponding control, except for iobitridol at 200 mgI/mL and ioxaglate at 50-200 mgI/mL. All iodinated radiographic contrast media showed ABTS radical-scavenging activity. This finding suggested that iobitridol, iodixanol, iohexol, ioxaglate, and isovue exhibited weak antioxidant properties. The antioxidant ability depended on the type of free radical production and the concentration of iodinated radiographic contrast media.
本研究揭示了用于诊断放射学的碘化放射造影剂的抗氧化特性。采用二苯基-(2,4,6-三硝基苯基)亚氨基铵（DPPH）、血浆铁还原能力（FRAP）和2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)（ABTS）分析方法，测定了五种碘化放射造影剂的抗氧化特性，这五种造影剂分别为碘比醇（西奈泰克）、碘克沙醇（威视派克）、碘海醇（欧乃派克）、碘克酸（六异泛影葡胺）和碘帕醇（碘必乐）。抗坏血酸和Trolox溶液用作阳性对照。使用分光光度计记录有色产物的吸光度强度。对于DPPH和ABTS分析，与对照相比，分别在533和752nm处的吸光度强度降低；这表明抗氧化活性增加。对于FRAP分析，与对照相比，593nm处的吸光度强度增加；这表明抗氧化活性增加。结果表明，与相应对照相比，五种碘化放射造影剂在DPPH自由基清除活性方面没有差异。除了200mgI/mL的碘比醇和50 - 200mgI/mL的碘克酸外，所有这些碘化放射造影剂的铁还原能力与相应对照相比也没有差异。所有碘化放射造影剂均表现出ABTS自由基清除活性。这一发现表明，碘比醇、碘克沙醇、碘海醇、碘克酸和碘帕醇具有较弱的抗氧化特性。抗氧化能力取决于自由基产生的类型和碘化放射造影剂的浓度。
