Naydenov Mladen, Gospodinova Nadezhda, Apostolova Elena, Anachkov Nikolay, Baev Vesselin, Gozmanova Mariyana, Minkov Ivan, Yahubyan Galina
Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv, Bulgaria.
Institute of Molecular Biology and Biotechnology, Plovdiv, Bulgaria.
Acta Biochim Pol. 2018;65(3):391-396. doi: 10.18388/abp.2017_1590. Epub 2018 Aug 27.
Genetic diversity caused by transposable element movement can play an important role in plant adaptation to local environments. Regarding genes, transposon-induced alleles were mostly related to gene bodies and a few of them to promoter regions. In this study, promoter regions of 9 stress-related genes were searched for transposable element insertions in 12 natural accessions of Arabidopsis thaliana. The promoter screening was performed via PCR amplification with primers designed to flank transposable element insertions in the promoter regions of the reference accession Col-0. Transposable element-associated insertion/deletion (indel) polymorphisms were identified in 7 of the 12 promoter loci across studied accessions that can be developed further as molecular markers. The transposable element absence in the promoter regions of orthologous genes in A. lyrata indicated that the insertion of these transposable elements in A. thaliana lineage had occurred after its divergence from A. lyrata. Sequence analysis of the promoter regions of CML41 (Calmodulin-like protein 41) and CHAP (chaperone protein dnaJ-related) confirmed the indel polymorphic sites in four accessions - Col-0, Wassilewskija, Shahdara, and Pirin. The observed indel polymorphism of the CHAP promoter region was associated with specific gene expression profiles in the different accessions grown at a normal and elevated temperature in a plant growth chamber. The collected data can be a starting point for gene expression profiling studies under conditions resembling the natural habitats of accessions.
由转座元件移动引起的遗传多样性在植物适应当地环境中可能发挥重要作用。关于基因，转座子诱导的等位基因大多与基因体相关，只有少数与启动子区域相关。在本研究中，在12个拟南芥自然种质中搜索了9个胁迫相关基因的启动子区域中的转座元件插入情况。通过PCR扩增进行启动子筛选，所用引物设计用于侧翼参考种质Col-0启动子区域中的转座元件插入。在研究的种质中，12个启动子位点中的7个鉴定出了与转座元件相关的插入/缺失（indel）多态性，这些多态性可进一步开发为分子标记。琴叶拟南芥直系同源基因启动子区域中转座元件的缺失表明，这些转座元件在拟南芥谱系中的插入发生在其与琴叶拟南芥分化之后。对CML41（类钙调蛋白41）和CHAP（伴侣蛋白dnaJ相关）启动子区域的序列分析证实了Col-0、瓦西列夫斯基、沙达拉和皮林这四个种质中的indel多态性位点。在植物生长室中，在正常温度和高温下生长的不同种质中，观察到的CHAP启动子区域的indel多态性与特定的基因表达谱相关。收集到的数据可以作为在类似于种质自然栖息地的条件下进行基因表达谱研究的起点。
