Sutliff V E, Raufman J P, Jensen R T, Gardner J D
Am J Physiol. 1986 Jul;251(1 Pt 1):G96-102. doi: 10.1152/ajpgi.1986.251.1.G96.
Vasoactive intestinal peptide and secretin increased cellular cAMP and pepsinogen secretion in dispersed chief cells from guinea pig gastric mucosa. With each peptide there was a close correlation between the dose-response curve for changes in cellular cAMP and that for changes in pepsinogen secretion. Vasoactive intestinal peptide-(10-28) and secretin-(5-27) had no agonist activity and antagonized the actions of vasoactive intestinal peptide and secretin on cellular cAMP and pepsinogen secretion. Studies of binding of 125I-vasoactive intestinal peptide and of 125I-secretin indicated that gastric chief cells possess four classes of binding sites for vasoactive intestinal peptide and secretin and that occupation of two of these classes of binding sites correlates with the abilities of vasoactive intestinal peptide and secretin to increase cellular cAMP and pepsinogen secretion. What function, if any, is mediated by occupation by the other two classes of binding sites remains to be determined.
血管活性肠肽和促胰液素可增加豚鼠胃黏膜分散主细胞内的环磷酸腺苷（cAMP）水平及胃蛋白酶原分泌。对于每种肽而言，细胞内cAMP变化的剂量反应曲线与胃蛋白酶原分泌变化的剂量反应曲线密切相关。血管活性肠肽-（10 - 28）和促胰液素-（5 - 27）无激动剂活性，并拮抗血管活性肠肽和促胰液素对细胞内cAMP及胃蛋白酶原分泌的作用。对125I - 血管活性肠肽和125I - 促胰液素结合的研究表明，胃主细胞拥有四类血管活性肠肽和促胰液素的结合位点，且占据其中两类结合位点与血管活性肠肽和促胰液素增加细胞内cAMP及胃蛋白酶原分泌的能力相关。另外两类结合位点的占据介导何种功能（若有）仍有待确定。
