H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Fitoterapia. 2018 Nov;131:168-173. doi: 10.1016/j.fitote.2018.08.016. Epub 2018 Aug 24.
Phytochemical investigation on the aerial parts of Lyonia ovalifolia (Wall.) Drude led to the isolation of three new iridoids, lyonofolin A (1), lyonofolin B (2), and lyonofolin C (3), and a known iridoid, gelsemiol (4). Structures of compounds 1-4 were determined by extensive spectroscopic analyses, including EI-MS, HREI-MS, UV, IR, and 1D- and 2D-NMR (HMBC, HSQC, COSY, NOESY) spectroscopic methods. The effect of insulin secretion of compounds 1, 2, and 4 were evaluated in mice pancreatic islets cellular model. This insulin secretory assay demonstrated that compound 2 potentiates glucose-induced insulin secretion, and thus can serve as a new insulin secretagogue for the treatment of diabetes. The newly isolated compounds were further evaluated against normal 3 T3 cell lines for cytotoxicity, where they did not show any cytotoxicity.
对腊莲绣球（Wall.）Drude 的地上部分进行植物化学研究，分离得到了三个新的环烯醚萜类化合物，即腊莲绣球苷 A（1）、腊莲绣球苷 B（2）和腊莲绣球苷 C（3），以及一个已知的环烯醚萜类化合物，钩吻素子（4）。通过 EI-MS、HREI-MS、UV、IR、1D 和 2D-NMR（HMBC、HSQC、COSY、NOESY）光谱方法等广泛的光谱分析确定了化合物 1-4 的结构。在小鼠胰岛细胞模型中评估了化合物 1、2 和 4 对胰岛素分泌的影响。该胰岛素分泌试验表明，化合物 2 增强了葡萄糖诱导的胰岛素分泌，因此可以作为治疗糖尿病的新型胰岛素分泌剂。对新分离的化合物进行了进一步的正常 3T3 细胞系的细胞毒性评估，它们没有表现出任何细胞毒性。
