Douvas G S, Berger E M, Repine J E, Crowle A J
Am Rev Respir Dis. 1986 Jul;134(1):44-8. doi: 10.1164/arrd.1986.134.1.44.
The effects of human monocyte maturity on the replication of virulent Mycobacterium tuberculosis were examined. Mycobacteria grew readily in freshly isolated, adherent peripheral blood monocytes and in monocyte-derived macrophages obtained after 7 days in culture, as measured by counts of acid-fast bacilli and colony-forming units. Monocytes cultured for only 3 days before infection, however, were less permissive for the mycobacteria than either uncultured or 7-day cells. The association between the low permissiveness of 3-day cells and superoxide production was examined. Mycobacteria induced only a slight increase in superoxide production during the first 60 min of infection in uncultured and in 3-day cells, and no increase in cells cultured for 7 days before infection. Freshly isolated adherent cells produced small amounts of superoxide in response to phorbol myristate acetate (PMA) stimulation, but PMA-induced superoxide production increased steadily for 7 days. Mycobacteria had no effect on superoxide production by PMA-stimulated adherent cells. These results suggest that the suppressive activity of the 3-day cells is not associated with the production of increased amounts of reactive oxygen species.
研究了人类单核细胞成熟度对强毒力结核分枝杆菌复制的影响。通过抗酸杆菌计数和菌落形成单位测定，结核分枝杆菌在新鲜分离的贴壁外周血单核细胞以及培养7天后获得的单核细胞衍生巨噬细胞中易于生长。然而，感染前仅培养3天的单核细胞对结核分枝杆菌的容许性低于未培养细胞或培养7天的细胞。研究了3天细胞低容许性与超氧化物产生之间的关联。在未培养细胞和3天细胞感染的最初60分钟内，结核分枝杆菌仅诱导超氧化物产生略有增加，而在感染前培养7天的细胞中则没有增加。新鲜分离的贴壁细胞对佛波醇肉豆蔻酸酯乙酸酯（PMA）刺激产生少量超氧化物，但PMA诱导的超氧化物产生在7天内稳步增加。结核分枝杆菌对PMA刺激的贴壁细胞的超氧化物产生没有影响。这些结果表明，3天细胞的抑制活性与活性氧物种产生量的增加无关。
