心房利钠多肽与自发性高血压大鼠（SHR）及易发生中风的SHR肾基底外侧膜的特异性结合。

Specific binding of atrial natriuretic polypeptide to renal basolateral membranes in spontaneously hypertensive rats (SHR) and stroke-prone SHR.

作者信息

Saito H, Inui K, Matsukawa Y, Okano T, Maegawa H, Nakao K, Morii N, Imura H, Makino S, Hori R

出版信息

Biochem Biophys Res Commun. 1986 Jun 30;137(3):1079-85. doi: 10.1016/0006-291x(86)90335-9.

Abstract

The binding of alpha-human atrial natriuretic polypeptide (alpha-hANP) to basolateral membranes isolated from renal cortex of spontaneously hypertensive rats (SHR) and stroke-prone SHR (SHR-SP) was measured at 0 degree C and compared with that of Wistar-Kyoto rats (WKY). The binding of 125I-alpha-hANP in SHR and SHR-SP at 14-15 weeks old demonstrated different binding profiles compared with that in WKY, though there were no apparent differences of the profiles among WKY, SHR and SHR-SP at 5 weeks old. For the high affinity binding sites, the apparent dissociation constant and the maximal binding capacity in SHR and SHR-SP were significantly decreased in comparison with those in WKY. The present data suggest that the altered characteristics of specific receptors for atrial natriuretic polypeptide in SHR and SHR-SP may be involved in the development or maintenance of genetic hypertension.

摘要

在0℃下测定了α-人心房利钠多肽（α-hANP）与自发性高血压大鼠（SHR）和易中风SHR（SHR-SP）肾皮质分离的基底外侧膜的结合，并与Wistar-Kyoto大鼠（WKY）进行了比较。14-15周龄的SHR和SHR-SP中125I-α-hANP的结合与WKY相比呈现出不同的结合模式，而5周龄时WKY、SHR和SHR-SP之间的结合模式没有明显差异。对于高亲和力结合位点，与WKY相比，SHR和SHR-SP中的表观解离常数和最大结合容量显著降低。目前的数据表明，SHR和SHR-SP中心房利钠多肽特异性受体特性的改变可能参与了遗传性高血压的发生或维持。