植物乳杆菌 C29 发酵大豆（DW2009）通过调节小胶质细胞激活和肠道微生物组成缓解 5XFAD 转基因小鼠的记忆损伤。
Lactobacillus plantarum C29-Fermented Soybean (DW2009) Alleviates Memory Impairment in 5XFAD Transgenic Mice by Regulating Microglia Activation and Gut Microbiota Composition.
机构信息
Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea.
DongWha Pharm Research Institute, 35-71, Topsil-ro, Giheung-gu, Yongin-Shi, Gyeonggi, 17084, Korea.
出版信息
Mol Nutr Food Res. 2018 Oct;62(20):e1800359. doi: 10.1002/mnfr.201800359. Epub 2018 Sep 11.
SCOPE
The study aims to determine whether Lactobacillus plantarum C29-fermented defatted soybean (FDS, DW2009) can attenuate memory impairment in 5XFAD transgenic (Tg) mice.
METHODS AND RESULTS
Oral administration of FDS or C29 increases cognitive function in Tg mice in passive avoidance, Y-maze, novel object recognition, and Morris water maze tasks. FDS or C29 treatment significantly suppresses amyloid-β, β/γ-secretases, caspase-3 expression, and NF-κB activation, and activates microglia and apoptotic neuron cell populations, and increases BDNF expression in the brain. FDS or C29 treatment suppresses blood and fecal lipopolysaccharide levels and Enterobacteriaceae population and increases lactobacilli/bifidobacteria populations.
CONCLUSION
FDS and C29 alleviates the decrease in cognitive function and inhibited amyloid-β expression in Tg mice by regulating microglia activation and gut microbiota composition.
范围
本研究旨在确定植物乳杆菌 C29 发酵脱脂大豆（FDS，DW2009）是否可以减轻 5XFAD 转基因（Tg）小鼠的记忆障碍。
方法和结果
FDS 或 C29 的口服给药可提高 Tg 小鼠在被动回避、Y 迷宫、新物体识别和 Morris 水迷宫任务中的认知功能。FDS 或 C29 处理可显著抑制淀粉样蛋白-β、β/γ-分泌酶、半胱天冬酶-3 的表达和 NF-κB 的激活，并激活小胶质细胞和凋亡神经元细胞群，增加大脑中的 BDNF 表达。FDS 或 C29 处理可抑制血液和粪便脂多糖水平和肠杆菌科种群，并增加乳杆菌/双歧杆菌种群。
结论
FDS 和 C29 通过调节小胶质细胞激活和肠道微生物群落组成，减轻 Tg 小鼠认知功能下降和抑制淀粉样蛋白-β表达。
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