Shin J, Ji I, Ji T H
Biochemistry. 1986 Jun 3;25(11):3410-5. doi: 10.1021/bi00359a047.
In order to examine the structure of the human choriogonadotropin (hCG) receptor and the follitropin (FSH) receptor on porcine granulosa cells, the hormone receptors were photoaffinity-labeled or affinity-cross-linked. The resulting hormone-receptor complexes were analyzed by alkaline cleavage of cross-links, reduction of disulfides, and peptide maps. The results revealed striking similarities in the structure of the hormone receptors. Both appear to be oligomeric; the hCG receptor has at least four components of 18, 24, 28, and 34 kDa, whereas the FSH receptor shows three distinct components of 18, 22, and 34 kDa. The 24- and the 22-kDa components are the sites for the primary photoaffinity labeling or affinity cross-linking by hCG and FSH, respectively. These components were linked by intercomponent disulfides. Reduction of cross-linked complexes revealed that in the hCG receptor the 24-, the 28-, and the 34-kDa components were disulfide-linked sequentially in a linear form as were the 22-, the 18-, and the 34-kDa components in the FSH receptor. The peptide maps of cross-linked hCG-receptor and FSH-receptor complexes, however, were distinct, indicating that the hCG receptor and the FSH receptor were not identical.
为了研究猪颗粒细胞上的人绒毛膜促性腺激素（hCG）受体和促卵泡激素（FSH）受体的结构，对激素受体进行了光亲和标记或亲和交联。通过交联的碱性裂解、二硫键的还原和肽图分析了所得的激素-受体复合物。结果显示激素受体的结构有显著相似性。两者似乎都是寡聚体；hCG受体至少有18、24、28和34 kDa的四个组分，而FSH受体显示18、22和34 kDa的三个不同组分。24 kDa和22 kDa的组分分别是hCG和FSH进行主要光亲和标记或亲和交联的位点。这些组分通过组分间二硫键相连。交联复合物的还原显示，在hCG受体中，24 kDa、28 kDa和34 kDa的组分以线性形式依次通过二硫键相连，FSH受体中的22 kDa、18 kDa和34 kDa的组分也是如此。然而，交联的hCG受体和FSH受体复合物的肽图是不同的，表明hCG受体和FSH受体并不相同。
