College of Animal Science and Technology, College of Life Sciences, Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China.
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Pest Manag Sci. 2019 Mar;75(3):844-851. doi: 10.1002/ps.5190. Epub 2018 Oct 5.
Fenoxaprop-ethyl (FE) is an active ingredient of commercially available herbicide formulations. Its overuse has caused much damage to the environment, livestock breeding, agricultural crops and humans. However, little is known about the effects of FE exposure on female reproductive health and the mechanisms underlying those effects. In this study, we investigated the toxic effects of FE on oocyte quality and their underlying mechanisms in mice fed a diet containing FE.
Ovary weight and numbers of oocytes were reduced in FE-treated mice. Moreover, oocyte quality was seriously impaired, as shown by the reduced rate of first polar body extrusion and fertilization ability in vivo. In FE-treated mice, oocytes presented reduced actin expression and abnormal meiotic spindle morphology, which indicate that cytoskeletal integrality is disrupted. Also, FE induced mitochondrial dysfunction, reflected by the accumulation of reactive oxygen species (ROS), apoptosis and autophagy, as revealed by fluorescent staining analysis and real-time polymerase chain reaction (qPCR). Finally, FE led to changes in epigenetic modifications such as histone H3K27me3 and H3K9me2 in oocytes.
Our results indicate that FE has adverse effects on oocyte quality as assessed by maturation and fertilization potential, due to disrupted cytoskeletal integrality, and mitochondrial dysfunction leading to ROS accumulation, apoptosis and autophagy. © 2018 Society of Chemical Industry.
精恶唑禾草灵（FE）是市售除草剂配方中的一种有效成分。其过度使用对环境、畜牧业、农作物和人类造成了极大的危害。然而，人们对 FE 暴露对女性生殖健康的影响及其潜在机制知之甚少。在本研究中，我们研究了 FE 对饮食中含有 FE 的小鼠卵母细胞质量的毒性作用及其潜在机制。
卵巢重量和卵母细胞数量减少。此外，卵母细胞质量严重受损，体内第一极体排出率和受精能力降低。在 FE 处理的小鼠中，卵母细胞的肌动蛋白表达减少，减数分裂纺锤体形态异常，表明细胞骨架完整性被破坏。此外，FE 诱导的线粒体功能障碍，通过荧光染色分析和实时聚合酶链反应（qPCR）显示活性氧（ROS）的积累、凋亡和自噬。最后，FE 导致卵母细胞中组蛋白 H3K27me3 和 H3K9me2 等表观遗传修饰的改变。
我们的结果表明，FE 通过破坏细胞骨架完整性和线粒体功能障碍导致 ROS 积累、凋亡和自噬，对卵母细胞的成熟和受精潜能等方面的质量产生不良影响。 © 2018 英国化学学会。
