Department of Polymer Science and Engineering, University of Massachusetts Amherst, Amherst, MA, 01003, USA.
Department of Veterinary and Animal Sciences, University of Massachusetts Amherst, Amherst, MA, 01003, USA.
Chem Asian J. 2018 Nov 16;13(22):3351-3365. doi: 10.1002/asia.201800849. Epub 2018 Aug 27.
Functional protein delivery has created new opportunities for studying intracellular processes and discovering new therapeutics. To that end, researchers have pursued intracellular protein delivery by using an increasing number of methods. This focus review will highlight polymeric carriers that non-covalently bind and deliver protein cargo in vitro. The correlation between polymer-protein binding and delivery as well as the correlation between complex-membrane binding and delivery is reviewed. Finally, binding and its relation to the intracellular function of the protein post-delivery is considered. The purpose of this review is to evaluate the role that binding interactions play in the non-covalent protein-delivery landscape. Presently, the literature does not adequately resolve how binding throughout the process ultimately affects delivery. The field does contain preliminary insights that are expected to impact future delivery applications when developed further.
功能蛋白递送为研究细胞内过程和发现新疗法创造了新的机会。为此，研究人员已经使用越来越多的方法来进行细胞内蛋白质的递送。本综述将重点介绍通过非共价键结合并在体外递送蛋白质货物的聚合物载体。综述了聚合物-蛋白质结合与递送之间的相关性，以及复合物与膜结合与递送之间的相关性。最后，还考虑了结合及其与蛋白质递送后细胞内功能的关系。本综述的目的是评估结合相互作用在非共价蛋白质递送领域中的作用。目前，文献还不能充分说明整个过程中的结合如何最终影响递送。该领域确实包含了一些初步的见解，这些见解有望在进一步发展时对未来的递送应用产生影响。
