Hassan H F, Coombs G H
Comp Biochem Physiol B. 1986;84(2):219-23.
A range of trypanosomatids (amastigotes and cultured promastigotes of Leishmania mexicana mexicana, cultured promastigotes of L. m. amazonensis, L. donovani and L. tarentolae, culture forms of Crithidia fasciculata, Herpetomonas muscarum muscarum and H. m. ingenoplastis and procyclic trypomastigotes of Trypanosoma brucei brucei) have been surveyed for the presence of purine- and pyrimidine-metabolising enzymes. Several common features were observed, including the presence of nucleosidases, catabolic phosphorylases, phosphoribosyltransferases, kinases and cytidine deaminase and the apparent absence of AMP deaminase, anabolic purine phosphorylase and cytosine deaminase. Significant differences between species were discovered, notably in adenine and adenosine metabolism. Nucleoside phosphotransferase active on inosine was detected in insect trypanosomatids but not in L. m. mexicana.
对一系列锥虫（墨西哥利什曼原虫墨西哥亚种的无鞭毛体和培养前鞭毛体、亚马逊利什曼原虫培养前鞭毛体、杜氏利什曼原虫、沙鼠利什曼原虫、克氏锥虫的培养形式、马斯喀特赫氏锥虫马斯喀特亚种和马斯喀特赫氏锥虫特化质体亚种以及布氏锥虫布氏亚种的前循环型锥鞭毛体）进行了嘌呤和嘧啶代谢酶的检测。观察到几个共同特征，包括核苷酶、分解代谢磷酸化酶、磷酸核糖基转移酶、激酶和胞苷脱氨酶的存在，以及明显不存在AMP脱氨酶、合成代谢嘌呤磷酸化酶和胞嘧啶脱氨酶。发现不同物种之间存在显著差异，尤其是在腺嘌呤和腺苷代谢方面。在昆虫锥虫中检测到对肌苷有活性的核苷磷酸转移酶，但在墨西哥利什曼原虫墨西哥亚种中未检测到。
