Ardaillou N, Nivez M P, Ardaillou R
FEBS Lett. 1986 Aug 18;204(2):177-82. doi: 10.1016/0014-5793(86)80807-9.
Recently a stimulatory effect of atrial natriuretic peptide (ANP) on the particulate guanylate cyclase system has been reported in the glomeruli from different species. Using cultures of homogeneous human glomerular cell lines, we found that rat and human ANP stimulated markedly cGMP formation in epithelial cells with a threshold dose of 1 nM. A 20-fold increase was obtained at 5 microM. Stimulation was also present but less substantial (2-fold at 5 microM) in mesangial cells. cGMP was formed rapidly and released in the medium. ANP and sodium nitroprusside, an activator of soluble guanylate cyclase, had additive effects on cGMP formation. ANP did not inhibit cAMP formation in both cell lines. These results demonstrate that, at least in the human species, epithelial cells represent the main target of ANP in the glomerulus. Synthesis of cGMP in the glomerular epithelial cells in response to ANP also suggests that the excess of urinary cGMP produced by the kidney which is observed after ANP administration is of glomerular rather than of tubular origin.
最近有报道称，心房利钠肽（ANP）对不同物种肾小球中的颗粒型鸟苷酸环化酶系统具有刺激作用。利用人肾小球细胞系的纯培养物，我们发现大鼠和人ANP能显著刺激上皮细胞中cGMP的生成，阈剂量为1 nM。在5 μM时增加了20倍。在系膜细胞中也存在刺激作用，但程度较轻（5 μM时为2倍）。cGMP迅速生成并释放到培养基中。ANP和可溶性鸟苷酸环化酶激活剂硝普钠对cGMP的生成具有相加作用。ANP在两种细胞系中均不抑制cAMP的生成。这些结果表明，至少在人类中，上皮细胞是肾小球中ANP的主要作用靶点。肾小球上皮细胞中响应ANP生成cGMP也提示，ANP给药后观察到的肾脏产生的尿cGMP过量源自肾小球而非肾小管。
