Krashennikova L V, Fedotov A R, Tarasov V A
Genetika. 1986 Jun;22(6):914-9.
Mutagenesis with Tn1 transposon was used to isolate mutants of ColE1 plasmid with inactivated gene responsible for immunity to colicin E1. Cells containing such mutants synthesized active colicin E1 and were sensitive to its action. Spontaneous and UV-induced colicin synthesis was strongly changed in the mutants, as compared to the control. Mutations occurring outside the immunity gene, including those within the structural gene for colicin E1, could also affect the immunity gene expression.
利用Tn1转座子诱变来分离大肠杆菌素E1免疫相关基因失活的ColE1质粒突变体。含有此类突变体的细胞合成了活性大肠杆菌素E1，并且对其作用敏感。与对照相比，突变体中自发的和紫外线诱导的大肠杆菌素合成发生了显著变化。发生在免疫基因之外的突变，包括那些在大肠杆菌素E1结构基因内的突变，也可能影响免疫基因的表达。
