兔肌肉中M型磷酸甘油变位酶的纯化

Purification of the M-type phosphoglyceromutase from rabbit muscle.

作者信息

Prehu C, Prehu M O, Kechemir D, Rosa R

出版信息

J Chromatogr. 1986 Jun 6;360(1):203-10. doi: 10.1016/s0021-9673(00)91663-7.

Abstract

A new method for the purification of M-type phosphoglyceromutase from rabbit muscle involving affinity chromatography on dye ligand media in the presence of 2,3-diphosphoglycerate is described. The method is rapid and technically simple. The purity of the enzyme was verified by electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulphate and by Cellogel electrophoresis. Immunological techniques showed that the anti-M antibodies are specific and do not cross-react with the B isozyme.

摘要

本文描述了一种从兔肌肉中纯化M型磷酸甘油变位酶的新方法，该方法是在2,3-二磷酸甘油酸存在的情况下，利用染料配体介质进行亲和色谱法。该方法快速且技术简单。通过在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳和Cellogel电泳验证了酶的纯度。免疫技术表明，抗M抗体具有特异性，不会与B同工酶发生交叉反应。