用于检测人血清抗HTLV-III抗体的对流免疫电泳

Counterimmunoelectrophoresis for detection of human serum antibody to HTLV-III.

作者信息

Blaser M J, Cohn D L, Cody H J, Penley K A, Judson F N, Saxinger W C, Weiss S H

出版信息

J Immunol Methods. 1986 Jul 24;91(2):181-6. doi: 10.1016/0022-1759(86)90477-1.

Abstract

We examined the usefulness of a counterimmunoelectrophoresis (CIE) technique for detecting antibodies to HTLV-III using sera that previously had been assessed for antibodies to HTLV-III by the standard enzyme-linked immunosorbent assay (ELISA). We selected a subset of 53 sera from patients with the acquired immune deficiency syndrome (AIDS) or the generalized lymphadenopathy syndrome (GLS) in which 81.1% were initially ELISA-positive, and 96.2% were positive by Western blot technique. In our standard HTLV-III CIE technique, 58.5% were positive and repeat testing increased the yield to 67.9%. Varying several parameters of the standard CIE assay did not improve sensitivity. We also studied 20 ELISA-negative and 10 ELISA-borderline sera from normal controls; all were negative by CIE. These results indicate that CIE may be used for detection of human serum antibodies to HTLV-III, but that the present assay was less sensitive than the HTLV-III ELISA.

摘要

我们使用先前已通过标准酶联免疫吸附测定（ELISA）评估过抗HTLV - III抗体的血清，研究了对流免疫电泳（CIE）技术检测抗HTLV - III抗体的实用性。我们从获得性免疫缺陷综合征（AIDS）或全身性淋巴结病综合征（GLS）患者中选取了53份血清样本，其中81.1%的样本最初ELISA检测呈阳性，96.2%的样本经Western印迹技术检测呈阳性。在我们的标准HTLV - III CIE技术中，58.5%的样本呈阳性，重复检测后阳性率提高到67.9%。改变标准CIE检测的几个参数并未提高检测灵敏度。我们还研究了来自正常对照的20份ELISA阴性血清和10份ELISA临界血清；所有样本经CIE检测均为阴性。这些结果表明，CIE可用于检测人血清中的抗HTLV - III抗体，但目前的检测方法不如HTLV - III ELISA灵敏。