Santa Catarina State University, Department of Food and Chemical Engineering, BR 282, Km 574, Pinhalzinho, SC 89870-000, Brazil.
Colorado State University, Department of Chemical & Biological Engineering, Polymer Physics & Engineering Laboratory, Fort Collins, CO 80523, USA.
Int J Biol Macromol. 2018 Dec;120(Pt A):522-528. doi: 10.1016/j.ijbiomac.2018.08.133. Epub 2018 Aug 27.
Arabic gum-based and chitosan-based hydrogels were synthesized through chemical crosslinking for the immobilization and controlled release of bovine serum albumin (BSA) and characterized by Fourier-transform infrared spectrometry, scanning electron microscopy and swelling assays. The degrees of swelling of the Arabic gum-based hydrogel were 13.22 and 22.95 g water per g dried hydrogel at pH 4.5 and 7.0, respectively, whereas the degrees of swelling of the chitosan-based hydrogel were 15.32 and 36.10 g water per g dried hydrogel, respectively. The water absorption mechanism in both hydrogels was non-Fickian, which involves diffusion through pores and macromolecular relaxation of the hydrophilic three-dimensional polymer network. BSA immobilization capacities of the Arabic gum-based and chitosan-based hydrogels after 240 min at pH 4.5 were 71.0 and 175.6 mg protein per g dried hydrogel, respectively. BSA immobilization capacities after 240 min at pH 7.0 were 62.5 and 154.2 mg protein per g dried hydrogel, respectively. The controlled release of BSA from the Arabic gum-based hydrogel was slightly more efficient than that of the chitosan-based hydrogel due to its more porous structure and weaker physiochemical interactions between the polymer network and protein molecule. Both hydrogels could be employed as carriers of proteins and as capsules for food supplements.
通过化学交联合成了基于阿拉伯胶和壳聚糖的水凝胶，用于固定和控制牛血清白蛋白（BSA）的释放，并通过傅里叶变换红外光谱、扫描电子显微镜和溶胀实验进行了表征。在 pH 值为 4.5 和 7.0 时，基于阿拉伯胶的水凝胶的溶胀度分别为 13.22 和 22.95 g 水/g 干水凝胶，而基于壳聚糖的水凝胶的溶胀度分别为 15.32 和 36.10 g 水/g 干水凝胶。两种水凝胶的吸水机制均为非菲克扩散，涉及通过孔和亲水三维聚合物网络的高分子松弛进行扩散。在 pH 值为 4.5 时，经过 240 分钟后，基于阿拉伯胶和壳聚糖的水凝胶对 BSA 的固定容量分别为 71.0 和 175.6 mg 蛋白质/g 干水凝胶。在 pH 值为 7.0 时，经过 240 分钟后，基于阿拉伯胶和壳聚糖的水凝胶对 BSA 的固定容量分别为 62.5 和 154.2 mg 蛋白质/g 干水凝胶。由于其更多孔的结构和聚合物网络与蛋白质分子之间较弱的物理化学相互作用，BSA 从基于阿拉伯胶的水凝胶中的控制释放效率略高于基于壳聚糖的水凝胶。这两种水凝胶都可以作为蛋白质载体和食品补充剂胶囊。
