Evola Christopher M, Hudson Tanner L, Huang Luping, Corbett Adrian M, Mayes Debra A
Department of Neuroscience, Cell Biology and Physiology, Wright State University, Boonshoft School of Medicine, College of Science and Math, Dayton, OH 45435, USA.
Aging (Albany NY). 2018 Aug 31;10(8):2148-2169. doi: 10.18632/aging.101538.
Recently epidemiological studies suggest females lose neuroprotection from neurodegenerative diseases as they go through menopause. It has been hypothesized that this neuroprotection is hormone-dependent. The current study characterized cell signaling molecules downstream of estrogen receptor beta that are known to play a role in memory, PKC, ERK, and connexin-43, in regions of the brain associated with memory decline in an attempt to elucidate significant changes that occur post-estrus. Total whole cell lysates were compared to isolated mitochondrial protein because mitochondrial function is known to be altered during aging. As hypothesized, protein concentrations differed depending on age, gender, and brain region. Additionally, many of these changes occurred within mitochondria but not within whole cell lysates indicating that these are epigenetic alterations. These findings accentuate the complexity of aging and provide insight into the gender-specific cellular processes that occur throughout this process.
最近的流行病学研究表明，女性在经历更年期时会失去对神经退行性疾病的神经保护作用。据推测，这种神经保护作用依赖于激素。当前的研究对雌激素受体β下游的细胞信号分子进行了表征，这些分子已知在记忆、蛋白激酶C（PKC）、细胞外信号调节激酶（ERK）和连接蛋白43中发挥作用，研究对象为大脑中与记忆衰退相关的区域，旨在阐明发情后期发生的显著变化。将全细胞裂解物与分离的线粒体蛋白进行比较，因为已知线粒体功能在衰老过程中会发生改变。正如所假设的那样，蛋白质浓度因年龄、性别和脑区而异。此外，许多这些变化发生在线粒体内，而不是全细胞裂解物中，这表明这些是表观遗传改变。这些发现突出了衰老的复杂性，并为整个过程中发生的性别特异性细胞过程提供了见解。
