Gagner M, Shizgal H M, Forse R A
Surgery. 1986 Aug;100(2):298-305.
The effect of interleukin-1 and interleukin-2 on lipolysis and the adrenergic control of lipolysis was studied. Biopsy specimens of human adipose tissue were incubated in media containing 3H-palmitate and 14C-glucose, and the ratio of these isotopes was used to determine adipocyte lipolysis. Isoproterenol, clonidine, and theophylline were used in the media to stimulate the beta 1- and alpha 2-receptors and the subreceptor mechanism, respectively. Interleukin-1 had no effect on basal lipolysis, and at maximal receptor stimulation, it had no effect on the adrenergic receptor control of lipolysis. Interleukin-2 had no effect on basal lipolysis or on the beta-adrenergic receptor. Interleukin-2 significantly (p less than 0.02) decreased the alpha 2-inhibition of lipolysis by 68%. The effect of interleukin-2 on the alpha-receptor was demonstrated to be a significant 45% decrease (p less than 0.03) in the receptor responsiveness (a measure of the postreceptor mechanism) with no alteration in receptor sensitivity (a measure of receptor number). This data suggest that interleukin-2 stimulates lipolysis by decreasing the alpha 2-adrenergic inhibition of hormone-sensitive lipase.
研究了白细胞介素 -1 和白细胞介素 -2 对脂肪分解及脂肪分解的肾上腺素能控制的影响。将人脂肪组织活检标本在含有 3H - 棕榈酸盐和 14C - 葡萄糖的培养基中孵育，这些同位素的比例用于确定脂肪细胞的脂肪分解。在培养基中使用异丙肾上腺素、可乐定和茶碱分别刺激β1 和α2 受体以及亚受体机制。白细胞介素 -1 对基础脂肪分解无影响，在最大受体刺激时，它对脂肪分解的肾上腺素能受体控制也无影响。白细胞介素 -2 对基础脂肪分解或β - 肾上腺素能受体无影响。白细胞介素 -2 显著（p < 0.02）降低了α2 对脂肪分解的抑制作用达 68%。白细胞介素 -2 对α受体的作用表现为受体反应性（一种受体后机制的指标）显著降低 45%（p < 0.03）而受体敏感性（一种受体数量的指标）无改变。这些数据表明白细胞介素 -2 通过降低α2 肾上腺素能对激素敏感性脂肪酶的抑制作用来刺激脂肪分解。
