Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, United States.
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, United States; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD 20742, United States.
Colloids Surf B Biointerfaces. 2018 Dec 1;172:197-206. doi: 10.1016/j.colsurfb.2018.08.033. Epub 2018 Aug 18.
Quorum sensing (QS) enables intercellular communication after bacterial cells sense the autoinducers have reached or exceeded a critical concentration. Selectively amplifying specific bacterial "quorum" activity at a lower cell density is still a challenge. Here, we propose a novel platform of immune magnetic nano-assembly to amplify specific bacterial QS signaling via improving the bioavailability of autoinducers-2 (AI-2, furanosyl borate) from sender (wide-type, WT cells) to receiver (reporter cells). Antibody coated magnetic nanoparticle (MNPAB) was fabricated with an average diameter of 12 nm and a specific surface area of 96.5 m/g. The distribution efficiency of the antibody on the surface was 25.8 μg/m of magnetic nanoparticles. It was found that more than 3 × 10 of K12 serotype Escherichia coli (E. coli) reporter or WT cells were collected using 1 mg fabricated MNPAB at a saturated condition. The MNPAB not only captured E. coli WT cells but also brought them into proximity of E. coli (CT104, pCT6+pET-DsRed) reporter cells via magnetic attraction. The amplified QS signaling of the reporter cells by this immune magnetic nano-assembly was approximately 3 times higher than the nature QS signaling in cell suspension at optical density (OD) 0.08. This study foresees potential applications in amplifying specific biological QS signals based on a preprogrammed design.
群体感应（QS）使细菌细胞在感应到自动诱导物达到或超过临界浓度后能够进行细胞间通讯。在较低的细胞密度下选择性地放大特定细菌的“群体感应”活性仍然是一个挑战。在这里，我们提出了一种新型的免疫磁性纳米组装平台，通过提高自动诱导物-2（AI-2，呋喃硼酸）从供体（野生型，WT 细胞）到受体（报告细胞）的生物利用度来放大特定的细菌 QS 信号。用抗体包被的磁性纳米颗粒（MNPAB）的平均直径为 12nm，比表面积为 96.5m/g。抗体在表面的分布效率为 25.8μg/m 的磁性纳米颗粒。结果发现，在饱和条件下，使用 1mg 制备的 MNPAB 可以收集超过 3×10 的 K12 血清型大肠杆菌（E. coli）报告或 WT 细胞。MNPAB 不仅可以捕获 E. coli WT 细胞，还可以通过磁引力将它们带到 E. coli（CT104，pCT6+pET-DsRed）报告细胞附近。与细胞悬浮液在光密度（OD）0.08 时的自然 QS 信号相比，这种免疫磁性纳米组装体使报告细胞的 QS 信号放大了约 3 倍。这项研究预见了基于预设设计放大特定生物 QS 信号的潜在应用。
