Department of STD Control, Institute of Dermatology, Chinese Academy of Medical Science & Peking Union Medical College, National Center for STD Control, Chi Centers for Disease Control and Prevention, Nanjing, China.
Department of Dermatology, Xuzhong Medical University Affiliated Hospital of Lianyungang, Lianyungang, China.
J Cell Biochem. 2018 Dec;119(12):10151-10164. doi: 10.1002/jcb.27352. Epub 2018 Sep 1.
Syphilis is a sexually transmitted disease caused by the infection of Treponema pallidum subspecies pallidum. T-helper type 17-related genes, vitamin D receptor (VDR) gene, and chemokine/chemokine receptor genes are crucial in microbial infection. A total of 16 single-nucleotide polymorphisms (SNPs) in eight genes (interleukin [IL]-17A, IL-17F, IL-23R, VDR, C-C motif chemokine ligand [CCL] 2, CCL5, C-C chemokine receptor [CCR] 2, and CCR5) were analyzed in 188 patients with syphilis and 216 healthy controls. The results showed a strong correlation of IL-17A rs2275913 (AA vs AG + GG: odds ratio [OR], 1.78; 95% confidence interval [CI], 1.09 to 2.92; P = 0.020; A vs G: OR, 1.33; 95% CI, 1.01 to 1.76; P = 0.043) and rs3819024 (GG vs AA + GA: OR, 1.76; 95% CI, 1.06 to 2.91; P = 0.028; G vs A: OR, 1.36; 95% CI, 1.03 to 1.80; P = 0.030) with syphilis. In haplotype analysis, IL-17A rs2275913A/rs3819024G showed a risk effect (OR, 1.38; 95% CI, 1.04 to 1.82; P = 0.026), whereas IL-17A rs2275913G/rs3819024A showed a protective effect (OR, 0.76; 95% CI, 0.57 to 0.998; P = 0.048). The expression levels of IL-17A messenger RNA (mRNA) in peripheral blood mononuclear cells and IL-17A secretion in plasma were further examined. No significant differences were found between patients with syphilis and healthy controls. The study also explored whether IL-17A rs2275913 and rs3819024 were associated with the expression of IL-17A mRNA and IL-17A secretion in patients with syphilis. Similar negative results were found. In conclusion, the polymorphisms of IL-17A rs2275913 and rs3819024 and the haplotype containing these two SNPs influenced the susceptibility to syphilis in a Han Chinese population.
梅毒是一种由苍白密螺旋体亚种感染引起的性传播疾病。T 辅助细胞 17 相关基因、维生素 D 受体（VDR）基因和趋化因子/趋化因子受体基因在微生物感染中至关重要。在 188 例梅毒患者和 216 例健康对照中，分析了 8 个基因（白细胞介素[IL]-17A、IL-17F、IL-23R、VDR、C-C 基序趋化因子配体[CCL]2、CCL5、C-C 趋化因子受体[CCR]2 和 CCR5）中的 16 个单核苷酸多态性（SNP）。结果表明，IL-17A rs2275913（AA 与 AG+GG：比值比[OR]，1.78；95%置信区间[CI]，1.09 至 2.92；P=0.020；A 与 G：OR，1.33；95%CI，1.01 至 1.76；P=0.043）和 rs3819024（GG 与 AA+GA：OR，1.76；95%CI，1.06 至 2.91；P=0.028；G 与 A：OR，1.36；95%CI，1.03 至 1.80；P=0.030）与梅毒密切相关。在单体型分析中，IL-17A rs2275913A/rs3819024G 显示出风险效应（OR，1.38；95%CI，1.04 至 1.82；P=0.026），而 IL-17A rs2275913G/rs3819024A 则表现出保护作用（OR，0.76；95%CI，0.57 至 0.998；P=0.048）。进一步检测了外周血单个核细胞中 IL-17A 信使 RNA（mRNA）的表达水平和血浆中 IL-17A 的分泌情况。梅毒患者和健康对照组之间无显著差异。该研究还探讨了 IL-17A rs2275913 和 rs3819024 是否与梅毒患者中 IL-17A mRNA 的表达和 IL-17A 分泌有关。同样得到了阴性结果。综上所述，IL-17A rs2275913 和 rs3819024 的多态性以及包含这两个 SNP 的单体型影响了汉族人群梅毒的易感性。
