Department of orthopedic, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Department of orthopedic, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Int Immunopharmacol. 2018 Nov;64:110-115. doi: 10.1016/j.intimp.2018.07.014. Epub 2018 Aug 29.
Abietic acid has been reported to have anti-inflammatory activity. However, whether abietic acid has anti-inflammatory effects against osteoarthritis (OA) remains unclear. The present study aimed to measure the anti-inflammatory effects of abietic acid on OA in vitro. Human osteoarthritis chondrocytes were pretreated with abietic acid 1 h before IL-1β treatment. The results showed that treatment of abietic acid significantly inhibited IL-1β-induced TNF-α, NO, PGE2 production, and COX-2 expression. Abietic acid also concentration-dependently suppressed MMP1, MMP3, and MMP13 production induced by IL-1β. Moreover, the increased phosphorylation levels of NF-κB p65 and IκBα were inhibited by the treatment of abietic acid. We also found that the expression of PPAR-γ was increased by abietic acid. The inhibition of abietic acid on TNF-α, NO, and PGE2 production were reversed by GW9662, the inhibitor of PPAR-γ. In conclusion, the study elucidated abietic acid suppressed IL-1β-induced inflammation in human osteoarthritis chondrocytes by activating PPAR-γ.
松香酸具有抗炎活性。然而，松香酸是否对骨关节炎 (OA) 具有抗炎作用尚不清楚。本研究旨在测量松香酸对 OA 的体外抗炎作用。在 IL-1β 处理前 1 小时用松香酸预处理人骨关节炎软骨细胞。结果表明，松香酸处理显著抑制了 IL-1β 诱导的 TNF-α、NO、PGE2 的产生和 COX-2 的表达。松香酸还浓度依赖性地抑制了 IL-1β 诱导的 MMP1、MMP3 和 MMP13 的产生。此外，NF-κB p65 和 IκBα 的磷酸化水平也被松香酸的处理所抑制。我们还发现，松香酸增加了 PPAR-γ 的表达。PPAR-γ 抑制剂 GW9662 逆转了松香酸对 TNF-α、NO 和 PGE2 产生的抑制作用。综上所述，该研究阐明了松香酸通过激活 PPAR-γ 抑制了人骨关节炎软骨细胞中 IL-1β 诱导的炎症。
