Department of Animal Science, Raleigh 27695.
Department of Population Health and Pathobiology, North Carolina State University, Raleigh 27695.
J Dairy Sci. 2018 Nov;101(11):10409-10413. doi: 10.3168/jds.2017-14279. Epub 2018 Aug 29.
Our objective was to assess the ability of 3 herbal products to eliminate experimentally induced Streptococcus uberis mastitis. These herbal products, also known as phytoceuticals, are used in organically managed dairy cattle to maintain or promote udder health. The products tested were an intramammary product, a topical product, and a product applied to the vulvar area. These products are not approved by the US Food and Drug Administration for treatment of mastitis but they are sold to enhance milk quality or for maintenance or improvement of udder health. Each of the products contains at least one component shown to have antibacterial activity. In this study, we successfully challenge-inoculated 25 lactating dairy cows maintained under organic conditions with an isolate of S. uberis. All challenged cows were positive for S. uberis by milk culture after challenge. When cows met predefined criteria indicating the presence of clinical mastitis, treatment with 1 of the 3 products was initiated based upon a predetermined random allocation. Culture of aseptically collected quarter milk samples was performed before, during, and following challenge with S. uberis. Eight, 8, and 9 cows received the intravulvar, intramammary, and topical treatments, respectively. Milk from all cows that were treated with phytoceuticals were culture-positive for S. uberis at every time point following treatment through 168 h following the last phytoceutical treatment. Based upon the presence of clinical signs and for humane reasons, 2 intravulvar-treated cows, 1 topical-treated, and 4 intramammary-treated cows received intramammary antibiotic therapy. We concluded that the phytoceuticals tested, as dosed and used in this trial, did not produce bacterial cures in S. uberis-induced mastitis.
我们的目的是评估 3 种草药产品消除实验性诱导的无乳链球菌乳腺炎的能力。这些草药产品，也称为植物药，用于有机管理的奶牛中以维持或促进乳房健康。测试的产品是一种乳腺内产品、一种局部产品和一种应用于外阴区域的产品。这些产品未经美国食品和药物管理局批准用于治疗乳腺炎，但它们被出售以提高牛奶质量或用于维持或改善乳房健康。这些产品中的每一种都至少含有一种具有抗菌活性的成分。在这项研究中，我们成功地用无乳链球菌的分离株对 25 头处于有机条件下的泌乳奶牛进行了挑战接种。所有受挑战的奶牛在挑战后通过牛奶培养均为无乳链球菌阳性。当奶牛符合表明存在临床乳腺炎的预定标准时，根据预定的随机分配开始使用 3 种产品中的 1 种进行治疗。在无乳链球菌挑战前后和期间，无菌收集的四分体牛奶样本进行培养。分别有 8、8 和 9 头奶牛接受了阴道内、乳腺内和局部治疗。在用植物药治疗的所有奶牛中，在用植物药治疗后的 168 小时内，每一时间点的牛奶都对无乳链球菌呈培养阳性。基于临床症状的存在和出于人道原因，2 头阴道内治疗的奶牛、1 头局部治疗的奶牛和 4 头乳腺内治疗的奶牛接受了乳腺内抗生素治疗。我们得出的结论是，在所测试的植物药中，按照本试验中的剂量和使用方法，不会产生对无乳链球菌诱导的乳腺炎的细菌治愈作用。
