Ho P T, King I, Sartorelli A C
Biochem Biophys Res Commun. 1986 Jul 31;138(2):995-1000. doi: 10.1016/s0006-291x(86)80594-0.
HL-60 promyelocytic leukemia cells were induced to differentiate along the granulocytic pathway by treatment with dimethylsulfoxide. A significant decrease in transferrin receptor specific mRNA was seen as early as 4 hr after exposure to inducer. Relative to untreated controls, transcript levels decreased 5-fold within 24 hr of exposure to dimethylsulfoxide and remained depressed throughout a 6-day treatment period. Reductions in receptor transcripts preceded the loss of surface receptor which in turn preceded the expression of the differentiated phenotype. The findings demonstrate that differentiation-associated regulation of the transferrin receptor can occur at the transcriptional level.
用二甲基亚砜处理HL-60早幼粒细胞白血病细胞，诱导其沿粒细胞途径分化。早在暴露于诱导剂4小时后，转铁蛋白受体特异性mRNA就显著减少。与未处理的对照相比，暴露于二甲基亚砜24小时内转录水平下降了5倍，并且在整个6天的处理期间一直保持较低水平。受体转录本的减少先于表面受体的丧失，而表面受体的丧失又先于分化表型的表达。这些发现表明，转铁蛋白受体的分化相关调节可发生在转录水平。
