Jahshan F, Ertracht O, Abu Ammar A, Ronen O, Srouji S, Apel-Sarid L, Eisenbach N, Atar S, Sela E, Gruber M
Translational Otolaryngology Research Lab, Department of Otolaryngology, Galilee Medical Center, Nahariya, Israel.
Eliachar Research Laboratory, Galilee Medical Center, Nahariya, Israel.
Int J Pediatr Otorhinolaryngol. 2018 Oct;113:4-10. doi: 10.1016/j.ijporl.2018.07.012. Epub 2018 Jul 10.
Laryngotracheal damage is a well-described complication of endotracheal intubation and animal models are essential for studying the underlying cellular injury cascade. This novel rat model is based on transoral intubation and aims to simulate the common clinical scenario of tube-related airway damage.
Prospective randomized control pilot study. 28 male Sprague-Dawley were randomly assigned into three groups: control, 3-h' intubation and 6-h' intubation. The animals were then euthanized and their laryngotracheal complexes sent for histological analysis. Epithelial damage, mucosal thickness and mucosal gland hypertrophy were reviewed.
Total of 13 control animals and 15 intubated animals. 10 intubated animals survived the study protocol. Loss of epithelial surface architecture including damage to the microscopic ciliary mechanism was a common feature amongst all intubated animals. Average mucosal thickness of the larynx (including vocal cords and subglottic area) was 143 ± 88 μm for control rats, 315 ± 101 μm for rats intubated 3 h and 574 ± 174 μm for rats intubated 6 h .This was a statistically significant difference. Average mucosal gland hypertrophy in the laryngeal subsite was 0.41 ± 0.5 in control rats, 1.4 ± 0.5 in rats intubated 3 h and 2.0 ± 0.0 for rats intubated 6 h (statistically significant difference). There was a clear difference between three and 6 h of intubation with poorer mucosal injury parameters for longer intubation.
We describe a novel rat-based animal model for simulating airway mucosal damage following transoral intubation. This animal model is easy to carry out, reproducible and involves containable animal mortality rates.
喉气管损伤是气管插管一种广为人知的并发症，动物模型对于研究潜在的细胞损伤级联反应至关重要。这种新型大鼠模型基于经口插管，旨在模拟与导管相关的气道损伤这一常见临床情况。
前瞻性随机对照试验性研究。28只雄性斯普拉格-道利大鼠被随机分为三组：对照组、3小时插管组和6小时插管组。然后对动物实施安乐死，并将其喉气管复合体送去进行组织学分析。评估上皮损伤、黏膜厚度和黏膜腺体肥大情况。
共有13只对照动物和15只插管动物。10只插管动物存活至研究方案结束。上皮表面结构丧失，包括微观纤毛机制受损，是所有插管动物的共同特征。对照组大鼠喉部（包括声带和声门下区域）的平均黏膜厚度为143±88μm，3小时插管大鼠为315±101μm，6小时插管大鼠为574±174μm。这具有统计学显著差异。喉部亚部位的平均黏膜腺体肥大情况在对照组大鼠中为0.41±0.5，3小时插管大鼠中为1.4±0.5，6小时插管大鼠中为2.0±0.0（具有统计学显著差异）。插管3小时和6小时之间存在明显差异，插管时间越长，黏膜损伤参数越差。
我们描述了一种新型的基于大鼠的动物模型，用于模拟经口插管后的气道黏膜损伤。这种动物模型易于实施、可重复，且动物死亡率可控。
