a Department of Chemistry and Chemical Technology , Vidyasagar University , Midnapore , India.
b Department of Chemistry , Aliah University , West Bengal , India.
J Biomol Struct Dyn. 2019 Aug;37(13):3536-3549. doi: 10.1080/07391102.2018.1518157. Epub 2018 Nov 18.
Nanomaterials in different form have been thoroughly used in the area of pharmaceutics and medicine for drug delivery. The large scale of nanoparticles (NPs) synthesis from plant extract is much safe, cheap and eco-friendly. Here, we demonstrated a new, one-step, ultra-fast biosynthesis of gold nanoparticles (sc-AuNPs, 19.54nm) by using aqueous leaf extracts as a reducing and capping agent. And also, we presented the synthesis of citrate capped gold nanoparticles (cit-AuNPs) of approximately same size (19.66 nm). These two NPs were characterized by UV-Visible, dynamic light scattering, transmission electron microscope and energy dispersive X-ray spectroscopy. Fourier transform infrared spectroscopy confirmed that the functional groups like OH, NH, OH of COOH and CO were contributed in the sc-AuNPs formation. The negative zeta potential (-20.5, -22.8 mV) established the stability and dispersion of the sc- and cit-AuNPs. The anti-bacterial activity of the sc- and cit-AuNPs were checked against (DH5-Alpha). Minimum inhibitory concentration was 2.4 and 3.0 nM, respectively for sc- and cit-AuNPs. The interaction study of the sc-AuNPs/cit-AuNPs-human serum albumin (HSA) system was done by UV-Visible absorbance, fluorescence, circular dichroism, time resolved fluorescence spectroscopy and the measurement of zeta potential. Absorbance, three dimensional fluorescence, synchronous fluorescence and circular dichroism spectroscopy showed a minor conformational change of HSA upon interaction with the sc-AuNPs compared to cit-AuNPs. The present comparative study will advance our knowledge about the binding mode, mechanism and conformational change of the protein upon interaction with green synthesized sc-AuNPs and cit-AuNPs. Communicated by Ramaswamy H. Sarma.
纳米材料以不同的形式在药物输送领域的药物制剂和医学中得到了广泛的应用。从植物提取物中大规模合成纳米颗粒（NPs）更加安全、廉价和环保。在这里，我们展示了一种新的一步超快合成金纳米颗粒（sc-AuNPs，19.54nm）的方法，使用水提植物叶提取物作为还原剂和稳定剂。此外，我们还合成了具有相似尺寸（19.66nm）的柠檬酸稳定金纳米颗粒（cit-AuNPs）。这两种 NPs 通过紫外-可见分光光度法、动态光散射、透射电子显微镜和能量色散 X 射线能谱进行了表征。傅里叶变换红外光谱证实了 OH、NH、COOH 和 CO 等功能基团在 sc-AuNPs 形成中的作用。负的 zeta 电位（-20.5、-22.8mV）表明了 sc-和 cit-AuNPs 的稳定性和分散性。sc-和 cit-AuNPs 的抗菌活性用 DH5-Alpha 进行了检测。sc-和 cit-AuNPs 的最低抑菌浓度分别为 2.4 和 3.0 nM。通过紫外-可见吸收光谱、荧光光谱、圆二色光谱、时间分辨荧光光谱和 zeta 电位测量，研究了 sc-AuNPs/cit-AuNPs-人血清白蛋白（HSA）系统的相互作用。吸收光谱、三维荧光、同步荧光和圆二色光谱表明，与 cit-AuNPs 相比，sc-AuNPs 与 HSA 相互作用时，HSA 的构象仅有轻微变化。这项比较研究将增进我们对绿色合成的 sc-AuNPs 和 cit-AuNPs 与蛋白质相互作用时的结合模式、机制和构象变化的认识。Ramaswamy H. Sarma 通讯。
