Department of Electrical Engineering, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Analyst. 2018 Sep 24;143(19):4674-4683. doi: 10.1039/c8an00093j.
Short-term acclimation response of individual cells of Thalassiosira weissflogii was monitored by Synchrotron FTIR imaging over the span of 75 minutes. The cells, collected from batch cultures, were maintained in a constant flow of medium, at an irradiance of 120 μmol m-2 s-1 and at 20 °C. Multiple internal reflections due to the micro fluidic channel were modeled, and showed that fringes are additive sinusoids to the pure absorption of the other components of the system. Preprocessing of the hyperspectral cube (x, y, Abs(λ)) included removing spectral fringe using an EMSC approach. Principal component analysis of the time series of hyperspectral cubes showed macromolecular pool variations (carbohydrates, lipids and DNA/RNA) of less than 2% after fringe correction.
通过同步辐射傅里叶变换成像，监测了个体海链藻细胞的短期驯化反应，持续时间为 75 分钟。从批量培养中收集的细胞在 120 μmol m-2 s-1 的辐照度和 20°C 的恒定介质流中维持。由于微流道而产生的多次内部反射进行了建模，结果表明条纹是系统其他组成部分的纯吸收的附加正弦曲线。对高光谱立方体 (x、y、Abs(λ))进行预处理，包括使用 EMSC 方法去除光谱条纹。高光谱立方体时间序列的主成分分析表明，条纹校正后，大分子库变化（碳水化合物、脂质和 DNA/RNA）小于 2%。
