Szulc-Musioł B, Dolińska B, Jasik K, Ryszka F
Department of Applied Pharmacy, School of Pharmacy and the Division of Laboratory Medicine, Medical University of Silesia, Katowice, Poland.
Department of Applied Pharmacy, School of Pharmacy and the Division of Laboratory Medicine, Medical University of Silesia, Katowice, Poland; Pharmaceutical Research and Production Plant Biochefa, Sosnowiec, Poland.
Transplant Proc. 2018 Sep;50(7):2229-2234. doi: 10.1016/j.transproceed.2018.02.135. Epub 2018 Mar 14.
The aim of the study was to assess the degree of liver damage in a rabbit perfused with histidine-tryptophan-ketoglutarate (HTK [Custodiol]) solution with and without the presence of prolactin (PRL) based on biochemical studies in perfundate and ultrastructural analysis of hepatocytes.
The experiment was carried out on rabbits. Liver ischemia was used in the study, based on Pringle's maneuver. About 70% of the rabbit liver lobes were perfused with HTK with or without the addition of PRL (2.5μg/g liver/h) under ischemic conditions for 2 hours. In the perfundate, the activity of enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), γ-glutamyl transpeptidase (GGT), and lactate concentration were determined. Liver biopsies were collected for histopathologic evaluation under an electron microscope.
The addition of PRL to the HTK significantly reduced the leakage of enzymes from the liver to perfundate compared with the control group without PRL. The activity of ALT, AST, LDH, and GGT in the perfundates obtained after 2-hour perfusion with HTK-PRL solution was lower when compared with activity of the same parameters determined in perfundates with liver perfused with HTK without PRL. The area under the curve (AUC) calculated for GGT, LDH, and lactates was significantly higher after perfusion with the HTK than with HTK with the addition of PRL. In the study group, bile was secreted throughout the whole experiment. The morphological confirmation of these results was obtained by means of transmission microscopy.
PRL added to the preservation solution significantly inhibits the process of liver cell cytolysis, which may suggest its hepatoprotective effect.
本研究旨在基于灌注液中的生化研究和肝细胞的超微结构分析，评估在有和没有催乳素（PRL）存在的情况下，用组氨酸 - 色氨酸 - 酮戊二酸（HTK [Custodiol]）溶液灌注的兔子的肝损伤程度。
实验在兔子身上进行。本研究采用基于普林格尔手法的肝脏缺血模型。在缺血条件下，约70%的兔肝叶用含或不含PRL（2.5μg/g肝脏/小时）的HTK灌注2小时。测定灌注液中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH）、γ-谷氨酰转肽酶（GGT）的活性以及乳酸浓度。采集肝脏活检组织进行电子显微镜下的组织病理学评估。
与未添加PRL的对照组相比，向HTK中添加PRL可显著减少肝脏中酶向灌注液的渗漏。用HTK - PRL溶液灌注2小时后获得的灌注液中，ALT、AST、LDH和GGT的活性低于用不含PRL的HTK灌注肝脏的灌注液中相同参数的活性。灌注HTK后计算的GGT、LDH和乳酸的曲线下面积（AUC）显著高于添加PRL的HTK。在研究组中，整个实验过程中均有胆汁分泌。通过透射显微镜对这些结果进行了形态学证实。
添加到保存液中的PRL可显著抑制肝细胞溶解过程，这可能表明其具有肝脏保护作用。
