N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospect 47, Moscow, 119991, Russia.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospect 47, Moscow, 119991, Russia.
Carbohydr Polym. 2018 Nov 15;200:1-5. doi: 10.1016/j.carbpol.2018.07.035. Epub 2018 Jul 26.
Two polysaccharides HT and HS were isolated from the sea cucumbers Holothuria tubulosa and Holothuria stellati, respectively. Purification of the natural polymers was performed by consecutive ion-exchange and gel permeation chromatography. The molar ratio of the monosaccharides and sulfate GlcA:GalNAc:Fuc:SONa was determined as 12:14:17:57 for HT and 12:12:16:60 for HS indicating that both polysaccharides are fucosylated chondroitin sulfates. Detailed structural characterization of HT and HS was performed using the 1D and 2D NMR spectroscopic methods. The main chain of HT and HS was shown to consist of alternating →4)- β-d-GlcA-(1→ and →3)-β-d-GalNAc-(1→ sequence, where the latter unit was sulfated at O-4 or both at O-4 and O-6. The ratio of GalNAc4S:GalNAc4S6S was determined as 1:4 for HT and 1:4.5 for HS. Three types of sulfated fucosyl branches Fucp2S4S, Fucp3S4S and Fucp4S attached to O-3 of glucuronic acid residues were found in a ratio of 3:3:1 for HT and 2:2:1 for HS.
两种多糖 HT 和 HS 分别从海参 Holothuria tubulosa 和 Holothuria stellati 中分离得到。天然聚合物的纯化通过连续离子交换和凝胶渗透色谱进行。单糖摩尔比和硫酸 GlcA:GalNAc:Fuc:SONa 分别为 12:14:17:57 用于 HT 和 12:12:16:60 用于 HS，表明这两种多糖都是岩藻糖基硫酸软骨素。使用 1D 和 2D NMR 光谱方法对 HT 和 HS 的详细结构进行了表征。HT 和 HS 的主链由交替的→4)-β-d-GlcA-(1→和→3)-β-d-GalNAc-(1→序列组成，其中后者单元在 O-4 或 O-4 和 O-6 处被硫酸化。GalNAc4S:GalNAc4S6S 的比例分别为 HT 的 1:4 和 HS 的 1:4.5。在 HT 中发现了三种类型的硫酸化岩藻糖基支链 Fucp2S4S、Fucp3S4S 和 Fucp4S，它们分别连接在葡萄糖醛酸残基的 O-3 上，比例为 3:3:1，而在 HS 中为 2:2:1。
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