N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 119991, Russia.
N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 119991, Russia.
Carbohydr Polym. 2020 Apr 15;234:115895. doi: 10.1016/j.carbpol.2020.115895. Epub 2020 Jan 20.
Fucosylated chondroitin sulfate (FCS) HeSp was isolated from the Patagonian sea cucumber Hemioedema spectabilis. Ion-exchange chromatography was applied for purification of the FCS from the crude extract of sulfated polysaccharides. Analysis of monosaccharide and sulfate content of HeSp revealed the molar ratio of GlcA:GalNAc:Fuc:SONa as 1.15:1:1.1:3.9. Molecular weight of HeSp (44.1 kDa) was determined by GPC. According to the NMR spectral data, the main fragment of HeSp was the trisaccharide →3)-β-d-GalNAc-(1→4)-β-d-GlcA(3-O-α-l-Fuc)-(1→, where GalNAc units were sulfated either at O-4, at O-6 or both at O-4 and O-6. The fucosyl branches attached to O-3 of GlcA showed also different patterns of sulfation: Fucp2S4S, Fucp4S and Fucp3S4S were found in a ratio of 3.8:1.5:1. Besides, small amounts of the disaccharide fragment →3)-β-d-GalNAc-(1→4)-β-d-GlcA3S-(1→ were observed in a structure of HeSp. The polysaccharide was found to block cancer cells adhesion to platelet-coated surface and to inhibit tubulogenesis, thus demonstrating the potential antitumor activity.
岩黄芪糖胺聚糖（FCS）HeSp 是从巴塔哥尼亚海参 Hemioedema spectabilis 中分离得到的。离子交换层析法被应用于从硫酸化多糖粗提物中纯化 FCS。HeSp 的单糖和硫酸根含量分析表明 GlcA:GalNAc:Fuc:SONa 的摩尔比为 1.15:1:1.1:3.9。HeSp 的分子量（44.1 kDa）通过 GPC 确定。根据 NMR 谱数据，HeSp 的主要片段是三糖→3）-β-d-GalNAc-（1→4）-β-d-GlcA（3-O-α-l-Fuc）-（1→，其中 GalNAc 单元在 O-4、O-6 或两者上被硫酸化。连接到 GlcA 的 O-3 的岩黄芪糖支链也显示出不同的硫酸化模式：Fucp2S4S、Fucp4S 和 Fucp3S4S 的比例为 3.8:1.5:1。此外，在 HeSp 的结构中还观察到少量的二糖片段→3）-β-d-GalNAc-（1→4）-β-d-GlcA3S-（1→。该多糖被发现可阻止癌细胞黏附在血小板包被的表面，并抑制管状形成，从而显示出潜在的抗肿瘤活性。
