Machala O, Mach O, Bubeník J, Hasek M
Folia Biol (Praha). 1986;32(3):178-82.
The presence of very high numbers of type C and type A retroviral particles was repeatedly confirmed not only in myeloma cells NSI, Ag 8 and in thymoma cells BW5147, EL 4 but also in B and T cell hybridomas constructed from the myeloma and thymoma cells used. Retroviral particles were demonstrated by current electron-microscopic and physicochemical methods. Biological tests for the induction of possible malignant or any pathological changes in the artificially infected sensitive cells during long-term cultivations in vitro or in vivo gave negative results. Nevertheless, on account of the hitherto unknown action of retroviruses one can suppose that monoclonal products from B and T cell hybridomas, particularly when used for practical purposes, should be purified because of their infectious nature.
不仅在骨髓瘤细胞NSI、Ag 8以及胸腺瘤细胞BW5147、EL 4中，而且在用这些骨髓瘤细胞和胸腺瘤细胞构建的B和T细胞杂交瘤中，都反复证实存在大量的C型和A型逆转录病毒颗粒。通过当前的电子显微镜和物理化学方法证实了逆转录病毒颗粒的存在。在体外或体内长期培养过程中，对人工感染的敏感细胞诱导可能的恶性或任何病理变化的生物学试验给出了阴性结果。然而，由于逆转录病毒迄今未知的作用，人们可以推测，B和T细胞杂交瘤的单克隆产物，特别是当用于实际目的时，因其具有感染性而应进行纯化。
