Gul Serdar Savas, Gurgul Serkan, Uysal Murat, Erdemir Fikret
Gaziosmanpaşa University, Faculty of Medicine, Department of Nuclear Medicine, Tokat 60100, Turkey.
Gaziantep University, Faculty of Medicine, Department of Biophysics, Gaziantep 27000, Turkey.
Urol J. 2018 Nov 17;15(6):387-396. doi: 10.22037/uj.v0i0.4404.
PURPOSE: The aim of the present study was to show the protective effect of pulsed magnetic field (PMF) application and melatonin administration on damage in testis in a one-sided torsion detorsion induced rat model using testicular scintigraphy with 99mTc pertechnetate, PET/CT with 18F-FDG and histopathological methods. MATERIALS AND METHODS: Sixty male rats were used in the study; 30 rats were randomly divided into five groups for one day applications of sham control, torsion, melatonin, pulsed magnetic field (PMF) and melatonin plus PMF. Similarly, for one week group, the other 30 rats were divided into the same five group (n=6), but the animals were sacrificed after one week. Rats were exposed to 50 Hz, 1 mT PMF for two hours. PET/CT with 37 MBq 18F-FDG and testicular scintigraphy with and 37 MBq 99mTc pertechnetate examinations were carried out, and testicular tissue was examined using histopathological methods. Results: In one day treatment, melatonin administration significantly increased perfusion and glucose metabolism compared to torsion group (p<0.01). Perfusion and glucose metabolism was also higher in the PMF and melatonin plus PMF groups than torsion group (p<0.01). In one week treatment, melatonin administration resulted in a significant higher perfusion rate and glucose metabolism rate compared to torsion group (p<0.01 and p<0.001, respectively). In addition, perfusion and glucose metabolism significantly increased in PMF and melatonin plus PMF groups compared to torsion group (p<0.01 and p<0.001, respectively). Furthermore, caspase-3 immunoreactivity and pathological changes increased in the torsion group (p<0.05). Melatonin and melatonin plus PMF treatment reduced the rate of immunoreactivity and pathological findings compared to the torsion group (p<0.05). CONCLUSION: According to these results it can be concluded that PMF application had a therapeutic benefit as effective as melatonin administering. In addition, it was indicated that PET/CT with 18F-FDG and testicular scintigraphy with 99mTc pertechnetate could be efficiently used in determining the treatment efficiency in testicular torsion.
目的：本研究旨在通过使用高锝[99mTc]酸盐进行睾丸闪烁扫描、18F-氟代脱氧葡萄糖正电子发射断层显像/计算机断层扫描（PET/CT）以及组织病理学方法，在单侧扭转复位诱导的大鼠模型中展示脉冲磁场（PMF）应用和褪黑素给药对睾丸损伤的保护作用。 材料与方法：本研究使用了60只雄性大鼠；30只大鼠被随机分为五组，分别用于假手术对照、扭转、褪黑素、脉冲磁场（PMF）以及褪黑素加PMF的一日应用。同样，对于一周组，另外30只大鼠被分为相同的五组（每组n = 6），但动物在一周后处死。大鼠暴露于50 Hz、1 mT的PMF下两小时。进行了37 MBq 18F-氟代脱氧葡萄糖的PET/CT以及37 MBq高锝[99mTc]酸盐的睾丸闪烁扫描检查，并使用组织病理学方法检查睾丸组织。结果：在一日治疗中，与扭转组相比，褪黑素给药显著增加了灌注和葡萄糖代谢（p < 0.01）。PMF组和褪黑素加PMF组的灌注和葡萄糖代谢也高于扭转组（p < 0.01）。在一周治疗中，与扭转组相比，褪黑素给药导致灌注率和葡萄糖代谢率显著更高（分别为p < 0.01和p < 0.001）。此外，与扭转组相比，PMF组和褪黑素加PMF组的灌注和葡萄糖代谢显著增加（分别为p < 0.01和p < 0.001）。此外，扭转组中半胱天冬酶-3免疫反应性和病理变化增加（p < 0.05）。与扭转组相比，褪黑素和褪黑素加PMF治疗降低了免疫反应性和病理结果的发生率（p < 0.05）。 结论：根据这些结果可以得出结论，PMF应用具有与褪黑素给药同样有效的治疗益处。此外，表明18F-氟代脱氧葡萄糖PET/CT和高锝[99mTc]酸盐睾丸闪烁扫描可有效地用于确定睾丸扭转的治疗效果。
West Afr J Med. 2007
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