Yurtçu Müslim, Abasiyanik Adnan, Avunduk Mustafa Cihat, Muhtaroğlu Sabahattin
Department of Pediatric Surgery, Meram Medical School of Selcuk University, Konya, Turkey.
J Pediatr Surg. 2008 Oct;43(10):1873-8. doi: 10.1016/j.jpedsurg.2008.01.065.
This study aimed to determine the effect of melatonin in preventing ischemia-reperfusion (I-R) injury-induced tissue damage and on spermatogenesis after experimental testicular torsion (TT).
Forty peripubertal rats were divided into 4 groups each containing 10 rats: control (C), sham (S), torsion plus detorsion (TD), and torsion plus melatonin (M). The left testes were rotated 720 degrees for 6 hours and detorsed for 6 hours thereafter. Serum inhibin B (IB) levels were measured in blood samples taken from all groups. Left orchiectomies were performed to determine the tissue levels of Johnsen's scores (JS) and malondialdehyde (MDA).
Serum IB levels in the S and TD groups were significantly lower compared with that in the C group, whereas they were higher in the M group compared with the TD group. The MDA levels were significantly lower in the C, S, and, M groups compared with the TD group. Johnsen's scores were significantly higher in the C, S, and M groups compared with the TD group.
Our results suggested that melatonin is a potent antioxidant agent in preventing testicular I-R injury, as shown by increased IB levels and JS.
本研究旨在确定褪黑素在预防实验性睾丸扭转（TT）后缺血再灌注（I-R）损伤诱导的组织损伤以及对精子发生的影响。
将40只青春期前大鼠分为4组，每组10只：对照组（C）、假手术组（S）、扭转加去扭转组（TD）和扭转加褪黑素组（M）。将左侧睾丸旋转720度持续6小时，之后去扭转6小时。从所有组采集血样测量血清抑制素B（IB）水平。进行左侧睾丸切除术以确定约翰森评分（JS）和丙二醛（MDA）的组织水平。
与C组相比，S组和TD组的血清IB水平显著降低，而与TD组相比，M组的血清IB水平更高。与TD组相比，C组、S组和M组的MDA水平显著降低。与TD组相比，C组、S组和M组的约翰森评分显著更高。
我们的结果表明，如IB水平和JS升高所示，褪黑素是预防睾丸I-R损伤的有效抗氧化剂。
