Ertekin T, Bilir A, Aslan E, Koca B, Turamanlar O, Ertekin A, Albay S
Department of Anatomy, University of Afyon Kocatepe, School of Medicine, Afyonkarahisar, Turkey.
Department of Histology and Embryology, University of Afyon Kocatepe, School of Medicine, Afyonkarahisar, Turkey.
Folia Morphol (Warsz). 2019;78(2):307-313. doi: 10.5603/FM.a2018.0080. Epub 2018 Sep 4.
Neural tube defects are congenital malformations of the central nervous system. Genetic predisposition and some environmental factors play an important role in the development of neural tube defects. This study aimed to investigate the effects of diclofenac sodium on the neural tube development in a chick embryo model that corresponds to the first month of vertebral deve- lopment in mammals.
Seventy-five fertile, specific pathogen-free eggs were incubated for 28 h and were divided into five groups of 15 eggs each. Diclofenac sodium was administered via the sub-blastodermic route at this stage. Incubation was continued till the end of the 48th h. All eggs were then opened and embryos were dissected from embryonic membranes and evaluated morphologically and histopathologically.
It was determined that the use of increasing doses of diclofenac sodium led to defects of midline closure in early chicken embryos. There were statistically significant differences in neural tube positions (open or close) among the groups. In addition; crown-rump length, somite number were significantly decreased in high dose experimental groups compared with control group.
This study showed that development of neurons is affected in chi- cken embryos after administration of diclofenac sodium. The exact teratogenic mechanism of diclofenac sodium is not clear; therefore it should be investigated.
神经管缺陷是中枢神经系统的先天性畸形。遗传易感性和一些环境因素在神经管缺陷的发生发展中起重要作用。本研究旨在探讨双氯芬酸钠对鸡胚模型神经管发育的影响，该模型相当于哺乳动物脊柱发育的第一个月。
75枚受精的无特定病原体鸡蛋孵化28小时，分为5组，每组15枚。在此阶段通过胚盘下途径给予双氯芬酸钠。继续孵化至第48小时结束。然后打开所有鸡蛋，从胚胎膜中取出胚胎，进行形态学和组织病理学评估。
确定使用递增剂量的双氯芬酸钠会导致早期鸡胚中线闭合缺陷。各组神经管位置（开放或闭合）存在统计学显著差异。此外，与对照组相比，高剂量实验组的顶臀长度、体节数显著减少。
本研究表明，给予双氯芬酸钠后鸡胚神经元发育受到影响。双氯芬酸钠的确切致畸机制尚不清楚，因此应进行研究。
