Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku , Tokyo 113-0033 , Japan.
Rigaku Corporation , 3-9-12 Matsubara-cho, Akishima-shi , Tokyo 196-8666 , Japan.
Org Lett. 2018 Sep 21;20(18):5606-5609. doi: 10.1021/acs.orglett.8b02284. Epub 2018 Sep 4.
By the genome-mining approach, a chimeric enzyme of prenyltransferase-diterpene synthase was discovered from Penicillium chrysogenum MT-12. Since its product exhibited broadened NMR signals, the structural determination by only the NMR analysis was difficult, but the crystalline sponge method successfully revealed the structure with a 6-5-5-5 fused ring system. This demonstrated that the collaboration between the genome-mining and crystalline sponge method has the potential to facilitate rapid inquiries into the unexplored chemical space of small molecules.
通过基因组挖掘方法，从青霉菌 MT-12 中发现了一种前体转移酶-二萜合酶的嵌合酶。由于其产物表现出拓宽的 NMR 信号，因此仅通过 NMR 分析进行结构测定较为困难，但晶体海绵方法成功揭示了具有 6-5-5-5 稠合环系统的结构。这表明基因组挖掘和晶体海绵方法的协作具有促进对小分子未探索化学空间进行快速研究的潜力。
