Wang X, Wang C, Li Q, Zhang J, Ji C, Sui J, Liu Z, Song X, Liu X
College of Life Science, Shandong Agriculture University, Taian, China.
College of Forestry, Shandong Agriculture University, Taian, China.
J Appl Microbiol. 2018 Dec 1;125(6). doi: 10.1111/jam.14099. Epub 2018 Sep 4.
The aim of this study was to identify efficient plant-beneficial rhizobacterium that has the potential to be developed as biocontrol agent for the control of wheat soil-borne diseases.
Rhizosphere soil samples were collected from a wheat field located in Taian City. Numerous bacteria were isolated and screened for antagonistic activity against soil-borne plant pathogenic fungi by performing dual-culture assays. Among them, XH-9 was selected for its highly antagonistic activity and others growth-promoting characteristics. Subsequently, the strain was identified as Bacillus amyloliquefaciens subsp. plantarum based on phylogenetic analysis of 16S rDNA sequence. Pot experiment indicated that XH-9 has good capacities for wheat, corn, and chili root colonization and considerably increased the biometric parameters of wheat seedlings. Quantitative real-time polymerase chain reaction experiments showed that the amount of Fusarium oxysporum associated with the XH-9 after treatment significantly decreased compared with control group.
Bacillus amyloliquefaciens subsp. plantarum XH-9 has the potential as biocontrol agent when applied in local arable land to prevent damage caused by F. oxysporum and other phytopathogens.
The development of biocontrol strategies for reducing the damage caused by plant pathogens is fully in accord with the current principles of sustainability.
本研究旨在鉴定高效的植物有益根际细菌，其有潜力被开发为防治小麦土传病害的生物防治剂。
从泰安市的一块小麦田采集根际土壤样本。通过进行双培养试验分离并筛选了许多细菌，以检测其对土传植物病原真菌的拮抗活性。其中，XH-9因其高度拮抗活性和其他促生长特性而被选中。随后，基于16S rDNA序列的系统发育分析，该菌株被鉴定为解淀粉芽孢杆菌植物亚种。盆栽试验表明，XH-9对小麦、玉米和辣椒根的定殖能力良好，并显著增加了小麦幼苗的生物量参数。定量实时聚合酶链反应实验表明，处理后与XH-9相关的尖孢镰刀菌数量与对照组相比显著减少。
解淀粉芽孢杆菌植物亚种XH-9应用于当地耕地时，有潜力作为生物防治剂来预防尖孢镰刀菌和其他植物病原体造成的损害。
开发减少植物病原体造成损害的生物防治策略完全符合当前的可持续发展原则。
